The MRL/MpJ mouse is an inbred laboratory strain of Mus musculus, known to exhibit enhanced autoimmunity, increased wound healing, and increased regeneration properties. We report the full-length mitochondrial DNA (mtDNA) sequence of the MRL mouse (Accession # EU450583), and characterize the discovery of two naturally occurring heteroplasmic sites. The first is a T3900C substitution in the TpsiCloop of the tRNA methionine gene (tRNA-Met; mt-Tm). The second is a heteroplasmic insertion of 1-6 adenine nucleotides in the A-tract of the tRNA arginine gene (tRNA-Arg; mt-Tr) at positions 9821-9826. Thelevel of heteroplasmy varied independently at these two sites in MRL individuals. The length of the tRNAArg A-tract increased with age, but heteroplasmy at the tRNA-Met site did not change with age. The finding of naturally occurring mtDNA heteroplasmy in an inbred strain of mouse makes the MRL mouse a powerful new experimental model for studies designed to explore therapeutic measures to alter the cellularburden of heteroplasmy.
Authors
- prof. dr hab. inż. Paweł Sachadyn link open in new tab ,
- Xiang-Ming Zhang,
- Lise Desquenne Clark,
- Robert K. Naviaux,
- Ellen Heber-Katz
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.mito.2008.07.007
- Category
- Publikacja w czasopiśmie
- Type
- artykuł w czasopiśmie z listy filadelfijskiej
- Language
- angielski
- Publication year
- 2008
