L-Glutamine: D-fructose-6-phosphate aminotransferase (EC 2.6.1.16), known also as glucosamine-6-phosphate (GlcN-6-P) syntase, catalyzes the first committed step in the amino sugar biosynthetic pathway in prokaryotic and eukaryotic organisms. The enzyme carries out a complex reaction involving ammonia transfer and sugar phosphate isomerization. GlcN-6-P syntase is an important point of metabolic control of amino sugar biosynthesis and postulated a putative target for antifungal chemotherapy. The enzyme is well known for its relative instability and difficulties in purification. One of the possible approaches to facilitate enzyme purification is a construction of its hexaHistag-containing version that can be easily isolated from complex mixtures by the one-step metal affinity chromatography. Unfortunately, introduction of hexahistydyl tags at either N- or C-terminus of GlcN-6-P synthase results in lost of the enzyme activity.
Authors
- Karolina Kwiatkowska,
- Justyna Czarnecka
Additional information
- Category
- Aktywność konferencyjna
- Type
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Language
- polski
- Publication year
- 2010
