The isolation of the components with anticipated biological properties i.e. antimicrobial, fungicidal, antioxidant and perhaps anticancer activity is for many years a one of the biggest issues of modern separation science. Their obtaining based on the isolation of the components with the biological activity from in vitro cultured plants by extraction techniques is often more difficult than their production by the chemical synthesis.The paper presents results of research on the development of the optimal conditions for the separation and identification of components from four carnivorous Droseraceae family plants extracts, such as D. muscipula, D. aliciae, D. binata and D. capensis. The main purpose of the research was to find the most selective chromatographic conditions to separate the naphthoquinones from flavonoids. In the paper the high performance liquid chromatography in the reversed phase (RP-HPLC), was used. The data obtained from separation plant extracts by isocratic elution, gradient elution and step elution was compared in the paper.The paper compares results obtained using gradient elution, isocratic elution and step elution. The use of the isocratic and step elution is favorable in the case of separation of the compounds mixture in the preparative scale chromatography (P-LC) and particularly in the process liquid chromatography. Application of the gradient elution is useful in the study of the plant metabolomics, identification and quantitation of the components of the plants extracts and also quality control and standardization of herbal materials.
Authors
Additional information
- Category
- Aktywność konferencyjna
- Type
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Language
- angielski
- Publication year
- 2011
