A new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific linker. Only DNA fragmens which consist pehX gene are amplified from the ligation product by PCR. Variety of PCR product length can differentiate the K. oxytoca strains among species.
Authors
Additional information
- Category
- Inne
- Type
- supllement, wydanie specjalne, dodatek
- Language
- angielski
- Publication year
- 2011
