The aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganism to the expression vectors in the Tabor-Studier system and its expression in Rosetta(DE3)pLysS Escherichia coli cells. The second phase of the study consisted of proteins purification using an initial denaturation of host proteins and salting-out proteins by ammonium sulfate.
Authors
Additional information
- Category
- Inne
- Type
- supllement, wydanie specjalne, dodatek
- Language
- angielski
- Publication year
- 2012
