A new perspective in the lipophilicity evaluation through RP-HPLC is permitted by analysis of the retentionfactor (k) obtained by injecting large volumes of test samples prepared in solvents immiscible withmobile phase. The experiment is carried out on representative groups of compounds with increasedtoxicity (mycotoxins and alkaloids) and amines with important biological activity (naturally occurringmonoamine compounds and related drugs), which are covering a large interval of lipophilicity. Thestock solution of each compound was prepared in hexane and the used mobile phases were mixturesof methanol or acetonitrile and water, in suited volume ratio. The injected volume was between 10 and100 L, while the used stationary phases were RP-18 and RP-8. On both reverse stationary phases theretention factors were linearly decreasing while the injection volume was increasing. In all cases, thelinear models were highly statistically significant. On the basis of the obtained results new lipophilicityindices were purposed and discussed. The developed lipophilicity indices and the computationallyexpressed ones are correlated at a high level of statistical significance.
Authors
- Costel Sarbu,
- Rodica Domnica Nascu-Briciu,
- Dorina Casoni,
- prof. dr hab. inż. Agata Kot-Wasik link open in new tab ,
- prof. dr hab. inż. Andrzej Wasik link open in new tab ,
- prof. dr hab. inż. Jacek Namieśnik link open in new tab
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.chroma.2012.10.007
- Category
- Publikacja w czasopiśmie
- Type
- artykuł w czasopiśmie wyróżnionym w JCR
- Language
- angielski
- Publication year
- 2012
