Publications Repository - Gdańsk University of Technology

Among nanomaterials, silver nanoparticles (AgNPs) have the broadest and most commercial applications due to their antibacterial properties, highlighting the need for exploring their potential toxicity and underlying mechanisms of action. Our main aim was to investigate whether AgNPs exert toxicity by inducing oxidative damage to DNA in human kidney HEK 293 cells. In addition, we tested whether this damage could be counteracted by plant extracts containing phytochemicals such as swertiamarin, mangiferin and homoorientin with high antioxidant abilities. We show that AgNPs (20nm) are taken up by cells and localised in vacuoles and cytoplasm. Exposure to 1, 25 or 100 µg/ml AgNPs leads to a significant dose-dependent increase in oxidised DNA base lesions (8-oxo-7,8-dihydroguanine or 8-oxoG) detected by the comet assay after incubation of nucleoids with 8-oxoG DNA glycosylase. Oxidised DNA base lesions and strand breaks caused by AgNPs were diminished by aqueous and methanolic extracts from both haulm and flower of Gentiana asclepiadea.

Authors

• Alexandra HUDECOVá,
• dr hab. inż. Barbara Kusznierewicz link open in new tab ,
• Elise RUNDéN-PRAN,
• Zuzana MAGDOLENOVá,
• KATARíNA HAĄPLOVá,
• Alessandra Rinna,
• Lise Marie Fjellsbø,
• Marcin Kruszewski,
• Anna Lankoff,
• Wiggo Johan Sandberg,
• Magne Refsnes,
• Tonje Skuland,
• Per Schwarze,
• Gunnar Brunborg,
• Magnar Bjøras,
• Andrew R. Collins,
• Eva MIADOKOVá,
• Eliąka GáLOVá,
• MáRIA DUĄINSKá