Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska- Dąbrowska A, Saunte DM, Arenderup MC, 2007, Fivehour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200–1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophytes DNA in pure cultures in less than 5 hours.
Authors
- Iwona Dąbrowska,
- Bożena Dworecka-Kaszak,
- dr hab. inż. Anna Brillowska-Dąbrowska link open in new tab
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.18388/abp.2014_1909
- Category
- Publikacja w czasopiśmie
- Type
- artykuł w czasopiśmie wyróżnionym w JCR
- Language
- angielski
- Publication year
- 2014
