Nowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field. Modifications based on fusion of DNA polymerases with DNA binding proteins is a novel and promising. In my study fusionTaq DNA polymerase showed increasing of resistance to inhibitors from clinical samples (lactoferrin) in comparison to unmodified Taq polymerase.
Authors
Additional information
- Category
- Aktywność konferencyjna
- Type
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Language
- angielski
- Publication year
- 2015
