Analytical procedure based on application of gas chromatography-mass spectrometry (GC-MS) at the step of detection, identification and quantitation of ibuprofen (IBU) and detection of its metabolites – 1-hydroxyibuprofen (1-OH IBU) and carboxyibuprofen (CBX IBU) in horse urine by selected ion monitoring mode after sample preparation covering extraction at pH 3-4 and methylation has been used. The ions at m/z 161, 220 (methylated ibuprofen), 178, 119 (methylated 1-hydroxyibuprofen) and 145, 264 (methylated carboxyibuprofen) was monitored to indicate the presence of investigated compounds in horse urine. The method was successfully applied to urine samples taken from two horses H1 and H2 that had been given a single oral dose of 0.9 and 1.1 mg/kg of ibuprofen respectively. The results of excretion of ibuprofen and its metabolites were reported.
Authors
- Emilia Waraksa link open in new tab ,
- dr Marzena Wójtowicz,
- dr inż. Dorota Kwiatkowska,
- prof. dr hab. inż. Jacek Namieśnik link open in new tab
Additional information
- Category
- Aktywność konferencyjna
- Type
- publikacja w wydawnictwie zbiorowym recenzowanym (także w materiałach konferencyjnych)
- Language
- angielski
- Publication year
- 2015
