Publications Repository - Gdańsk University of Technology

Biological systems, especially bacteriophages and peptides, are an attractive green alternative to other known methods of nanoparticle synthesis. In this work, for the first time, bacteriophages were employed to synthesize a specific peptide, capable of producing nanoparticles (NPs). Derivatives of M13 bacteriophage exposing a ZnO-binding peptide (TMGA-LGLKWPV) on either pIII or pVIII phage coat protein were constructed and used as a biotemplate. The exposition of the ZnO-binding peptide, synthesized by phages during their propagation in bacteria, on M13 virions provided a groundwork for growing ZnO nanostructures. Depending on the recombinant phage type used (M13-pIII-ZnO or 13-pVIII-ZnO), well separated ZnO NPs or complex 3D structures of ZnO NPs of ca. 20−40 nm were synthesized at room temperature. The synthesized ZnO nanoparticles served as a luminescent material that emitted light near the short wavelength end of the visible region (at ca. 400 nm). The next very low intensity emission band at 530 nm demonstrated that the ZnO material obtained is characterized by a low concentration of surface defects.

Authors

• dr hab. inż. Kamila Sadowska link open in new tab ,
• Joanna Karczewska-Golec,
• dr hab. inż. Jakub Karczewski link open in new tab ,
• Marcin Łoś,
• prof. dr hab. inż. Andrzej Kłonkowski link open in new tab ,
• Grzegorz Węgrzyn link open in new tab ,
• Piotr Golec