Human breast milk (HBM) is a complex biofluid containing components which are essential for the proper growth of the newborn (for example nutrients such as carbohydrates, proteins, lipids) and also bioactive substances. According to the literature, most of studies involving HBM nutrients investigations focused essentially on the determination of proteins. The molecular composition of lipids in HBM is not well known as the protein content. Until now, only few studies involving lipidomic analysis of HBM were performed. Moreover, most of them have been carried out in targeted and focused manner. Therefore, the aim of this study was to develop methodology for untargeted lipidomic analysis of HBM. Analysis of HBM lipids was carried out with use high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). Herein, methods for sample preparation, separation and identification of total lipids present in HBM during one analytical cycle is presented.
Authors
Additional information
- Category
- Aktywność konferencyjna
- Type
- materiały konferencyjne indeksowane w Web of Science
- Language
- angielski
- Publication year
- 2016
