Organotrophic anaerobic ammonium oxidation (anammox) bacteria can utilize small volatile fatty acids with nitrate as electron acceptors with less energy consumption and no biomass production. To achieve a faster and stable start-up of organotrophic anammox process, in this study, the growth of organotrophic anammox bacteria seeded from hybrid constructed wetland (CW) sediment under different TOC/TN ratios and different chloramphenicol concentrations were investigated. The incubation study was conducted at the TOC/TN ratio = 0.0375–0.1 or 0.1–0.2 for the period of over five months by using serum bottles. The anammox bacteria revealed a higher activity when the TOC/TN ratio was 0.1, with the removal efficiency of NH4+-N (60–80%) and NO2−-N (~100%). The relative abundances of anammox in the incubated CW sediment were about 30% higher in comparison with the municipal waste water treatment plant sludge, suggesting the CW sediment could be a viable source for the enrichment of organotrophic anammox bacteria. On the contrary, the continuous addition of 50 mg/L chloramphenicol completely inhibited the anammox activity in our study. Following the results of the batch tests, Candidatus Brocadia caroliniensis was successfully enriched with the CW sediment in an auto-controlled SBR for the period of 40 days.
Authors
- Xuejiao Yin,
- Jun Zhai,
- Wei Hu,
- Yue Li,
- Md. Hasibur Rahaman,
- prof. dr hab. inż. Jacek Mąkinia link open in new tab
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.ecoleng.2019.07.039
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2019
