Objective: This study evaluated the use of novel peptides derived from platelet-derived growth factor (PDGF-BB) as potential wound healing stimulants. One of the compounds (named PDGF2) was subjected for further research after cytotoxicity and proliferation assays on human skin cells. Further investigation included evaluation of: migration and chemotaxis of skin cells, immunological and allergic safety, the transcriptional analyses of adipose-derived stem cells (ASCs) and dermal fibroblasts stimulated with PDGF2, and the use of dorsal skin wound injury model to evaluate the effect of wound healing in mice. Approach: Colorimetric lactate dehydrogenase and tetrazolium assays were used to evaluate the cytotoxicity and the effect on proliferation. PDGF2 effect on migration and chemotaxis was also checked. Immunological safety and allergic potential were evaluated with a lymphocyte activation and basophil activation test. Transcriptional profiles of ASCs and primary fibroblasts were assessed after stimulation with PDGF2. Eight-week-old BALB/c female mice were used for dorsal skin wound injury model. Results: PDGF2 showed low cytotoxicity, pro-proliferative effects on human skin cells, high immunological safety, and accelerated wound healing in mouse model. Furthermore, transcriptomic analysis of ASCs and fibroblasts revealed the activation of processes involved in wound healing and indicated its safety. Innovation: A novel peptide derived from PDGF-BB was proved to be safe drug candidate in wound healing. We also present a multifaceted in vitro model for the initial screening of new compounds that may be potentially useful in wound healing stimulation. Conclusion: The results show that peptide derived from PDGF-BB is a promising drug candidate for wound treatment.
Authors
- Milena Deptuła,
- Przemysław Karpowicz,
- Anna Wardowska,
- dr inż. Piotr Sass link open in new tab ,
- dr inż. Paweł Sosnowski link open in new tab ,
- Alina Mieczkowska,
- Natalia Filipowicz,
- Maria Dzierżyńska,
- Justyna Sawicka,
- Ewa Nowicka,
- Paulina Langa,
- mgr Adriana Schumacher,
- Mirosława Cichorek,
- dr hab. Jacek Zieliński,
- Karolina Kondej,
- Franciszek Kasprzykowski,
- dr Artur Czupryn,
- dr Łukasz Janus,
- dr hab. Piotr Mucha,
- prof. dr hab. Piotr Skowron,
- dr hab. Arkadiusz Piotrowski,
- prof. dr hab. inż. Paweł Sachadyn link open in new tab ,
- dr hab. Sylwia Rodziewicz-Motowidło,
- dr hab. med. Michał Pikuła
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1089/wound.2019.1051
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2020
