Publications Repository - Gdańsk University of Technology

In this paper, various passivation schemes were applied at few-layer black phosphorus (FLBP) to achieve covalent functionalisation with 4-azidobenzoic acid, improving its electrochemical response intended for analytical and biosensing applications. The thermal and microwave assisted modification procedures in toluene and dime-thylformamide resulted in high reversibility of reactions on functionalised FLBP using a ferricyanide/ferrocya-nide redox probe. The lowest peak-to-peak separation of 91 mV, and high kinetics were obtained by thermal synthesis in dimethylformamide. Attachment of a =N-phenylene-COOH moiety to the FLBP limits its degradation under ambient conditions delivering a linker for a peptide bond with proteins in the –NH2 groups. The functionalised FLBP was applied for impedimetric detection of the Haemophilus Influenzae (HI) bacterial protein with a low limit of detection (LOD) of 5.82 μg mL-1 along with high sensitivity equal to 1.267% μg-1 mL. The proposed strategy delivers a novel phosphorene-based electrode for sensitive detection of various bacterial pathogens.

Authors

• dr inż. Paweł Jakóbczyk link open in new tab ,
• Marcin Kowalski link open in new tab ,
• Mateusz Brodowski link open in new tab ,
• dr inż. Anna Dettlaff link open in new tab ,
• dr inż. Bartłomiej Dec link open in new tab ,
• Dr Dawid Nidzworski,
• dr hab. inż. Jacek Ryl link open in new tab ,
• prof. dr hab. inż. Tadeusz Ossowski,
• dr hab. inż. Robert Bogdanowicz link open in new tab