Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce themortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFMresults approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88%relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seempromising for future surveys as potential platforms to be integrated into biosensors.
Authors
- Navid Rabiee,
- Yousef Fatahi,
- Sepideh Ahmadi,
- Nikzad Aabbariki,
- Amirhossein Ojaghi,
- Mohammad Rabiee,
- Fatemeh Radmanesh,
- Rassoul Dinarvand,
- Mojtaba Bagherzadeh,
- Ebrahim Mostafavi,
- Milad Ashrafizadeh,
- Pooyan Makvandi,
- Eder C. Lima,
- dr inż. Mohammad Saeb link open in new tab
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.scitotenv.2022.153902
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2022
