Lipidomics is a field of study aiming at a comprehensive analysis of a set of lipids in biological systems. This includes both quantitative and qualitative characterization of lipid profiles, comparative studies of lipidomes, and analysis of lipid-protein interactions. Lipids are structurally diversified that make their analysis a challenging task. The constant advances in analytical technologies, especially in the field of high-performance liquid chromatography and mass spectrometry, drive progress in lipid science. Commonly, lipidomics strategies are classified as targeted, untargeted, and focused. Many analytical techniques can be employed in the lipidomic study. The choice of an appropriate analytical tool for lipid analysis depends mainly on the aim of the study. In this chapter, we present an overview of the analytical techniques and data treatment used in the analysis of lipids in biological samples, with a special focus on LC-MS-based approaches. We also present the application of the lipidomic study in the comprehensive comparative analysis and discuss the limitation and advantages of the sample preparation techniques used in lipidomics.
Authors
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1007/978-3-030-63957-0_7-1
- Category
- Publikacja monograficzna
- Type
- rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
- Language
- angielski
- Publication year
- 2021
