Β-asarone is a phenylpropane derivative present in the rhizomes of Acorus calamus, that was proved to exhibit toxic effects in humans. Because of its presence the whole plant that is commonly used in traditional medicine for its sedative, anti-inflammatory, neuroprotective and other properties has limited application nowadays. In the study, qualitative and quantitative analysis of a collection of nine essential oil (EO) samples of European and Asian origin was performed. The final content of β-asarone in the tested samples ranged between 0.265 and 1.885 mg/mL. Having in mind a possible application of the EO as a biopesticide, this research aimed at the development of CPC-based purification protocol that could help remove β-asarone from EO. It was proved that the biphasic solvent system composed of n-hexane/EtOAc/MeOH/water, 9:1:9:1 (v/v/v/v) was capable of the removal of the toxic constituent in the CPC chromatograph operated in the ascending elution mode with 2200 rpm and a flow rate of 5 mL/min. The chromatographic analysis that lasted only 144 min effectively separated β-asarone (purity of 95.5%) and α-asarone (purity of 93.7%) directly from the crude Acorus calamus rhizome EO.
Authors
- dr inż. Paweł Szczeblewski link open in new tab ,
- Mateusz Wróblewski,
- dr inż. Julia Borzyszkowska-Bukowska link open in new tab ,
- Tetiana Bairamova link open in new tab ,
- Justyna Górska link open in new tab ,
- dr hab. inż. Tomasz Laskowski link open in new tab ,
- Anna Samulewicz,
- Michał Kosno link open in new tab ,
- Łukasz Sobiech,
- Justyna Polit,
- Wirginia Kukula-Koch
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1038/s41598-022-26726-6
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2022
