Manganese-dependent anaerobic oxidation of methane (Mn-AOM) is a major methane sink and vital to mitigating global warming. However, it is difficult for microorganisms to mediate electron transfer between the hardly dissolved CH4 and insoluble Mn(IV) minerals, leading to poor understanding of species mediating Mn-AOM. This study successfully enriched an anaerobic consortium mediating AOM driven by Mn-dependent respiratory growth, and for the first time, revealing a syntrophic pathway for Mn-AOM. The Mn-AOM occurrence was confirmed by long-term bioreactor performance and 13C-labelling batch experiment. Metagenomic and metatranscriptomic analyses demonstrated that the Candidatus Methanoperedens sp. BLZ1 was responsible for CH4 oxidation. The Luteitalea pratensis mediated extracellular electron transfer crossing S-layer of Ca. M. BLZ1 by conductive pili, and mediated microbial Mn(IV) reduction via multi-heme c-type cytochromes. This study offers an alternative syntrophic pathway for Mn-AOM by a microbial consortium instead of previously reported pathway by ANME alone. These outcomes provided new insight into migrating global climate change and manganese cycles.
Authors
- Wenbo Liu,
- Sai Xu,
- Hongpu Ma,
- Yuanyuan Li,
- prof. dr hab. inż. Jacek Mąkinia link open in new tab ,
- Jun Zhai
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.cej.2023.143478
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2023
