Publications Repository - Gdańsk University of Technology

Two non-platinum compounds, organometallic half-sandwich complexes [(η 5 -Cp)IrCl(L)]PF 6 (1) and [(η 5 -Cp)RhCl(L)]PF 6 (2) were prepared by treating pentamethylcyclopentadienyl chloride dimers of iridium(III) or rhodium(III) with the obtained 4-amino-N-(2,2'-bipyridin-5- yl)benzenesulfonamide ligand (L) and ammonium hexafluorophosphate. The crystal structures of ligand (L) and complexes 1 and 2 were obtained and then analyzed. Coordination reactions of the bipyridylsufonamide ligand (L) to the central ions were confirmed by UV- Vis, FT-IR, NMR ( 1 H, 13 C, and 15 N) spectroscopies, and stability study. Moreover, the interactions between the sulfaligand, Ir(III) and Rh(III) complexes with carbonic anhydrase (CA), human serum albumin (HSA) and also with CT-DNA have been investigated. Additionally, the reactivity of complexes toward GSH and NADH has been spectrophotometrically tested. A low affinity of complex 2 to GSH seems to be beneficial regarding its potential cytotoxic activity. The obtained compounds were then evaluated for their antiproliferative activity against four cancer cell lines from different origins: non-small cell lung cancer A549, colon cancer HCT-116, breast cancer MCF7, lymphoblastic leukemia Nalm-6, and a nonmalignant human embryonic kidney cell line HEK293. It has been observed that the iridium(III) complex (1) was found to exhibit a lack of antiproliferative properties against the tested cancer cell lines. The sulfonamide ligand (L) and the rhodium(III) complex (2) were studied in detail for colon cancer HCT-116 cell line, and the detailed biological analysis was reported and discussed.

Authors

• mgr Mateusz Kowalik,
• dr Joanna Masternak,
• mgr Mateusz Olszewski link open in new tab ,
• dr inż. Natalia Maciejewska link open in new tab ,
• dr hab. Katarzyna Kazimierczuk link open in new tab ,
• Jerzy Sitkowski,
• Aleksandra Dąbrowska,
• Agnieszka Chylewska,
• Mariusz Makowski