In this study, a vortex assisted magnetic deep eutectic solvent based liquid phase microextraction (VA-MDES-LPME) technique was developed for the extraction of patulin prior to its determination by ultraviolet–visible spectrophotometry (UV–Vis). For this purpose, the use of a magnetic deep eutectic solvent (MDES) consisting of trihexyltetradecylphosphonium chloride, MnCl2, and octanoic acid (molar ratio 1:2:2) was tested to perform an efficient extraction. Important experimental parameters (MDES-3 vol, pH, vortex time, and ethanol volume) were optimized using Box-Behnken Design. Under optimum conditions (MDES-3 vol 560 µL, pH 3.5, vortex time 300 s, and ethanol volume 300 µL), the limits of detection for the model solution and matrix-matched solution were 0.06 ng mL−1 and 0.37 ng mL−1, and the calibration ranges were 0.2–400 ng mL−1 and 1.2–300 ng mL−1, respectively. Furthermore, the extraction recovery was calculated to be 96.3–97.4 % with a low relative standard deviation (2.1–2.5 %). The VA-MDES-LPME technique was successfully applied for the determination of patulin from fruit and juice samples with an enrichment factor between169 and 185.
Authors
- Hameed Ul Haq,
- dr hab. inż. Grzegorz Boczkaj link open in new tab ,
- Nail Altunay
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/j.microc.2024.112472
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2025
