Repozytorium publikacji - Politechnika Gdańska

The objective of this paper is to provide information about application of solid phase extraction (SPE) for isolation of nine high-intensity sweeteners (acesulfame-K, alitame, aspartame, cyclamate, dulcin, neotame, saccharin, sucralose and neohesperidin dihydrochalcone) from aqueous solutions. The influence of several types of LC-MS compatible buffers (different pH values and compositions) on their recovery has been studied and discussed. A number of commercially available SPE cartridges, such as Chromabond C18ec, Strata-X RP, Bakerbond Octadecyl, Bakerbond SDB-1, Bakerbond SPE Phenyl, Oasis HLB, LiChrolut RP-18, Supelclean LC-18, Discovery DSC-18 and Zorbax C18 were tested in order to evaluate their applicability for isolation of analytes. Very high recoveries (better than 92%) of all studied compounds were obtained using formic acid - N,N-diisopropylethylamine buffer adjusted to pH 4.5 and C18-bonded silica sorbents. Behaviour of polymeric sorbents strongly depends on their structure. Strata-X RP behaves much like a C18-bonded silica sorbent. Recoveries obtained using Oasis HLB were comparable with those observed for silica based sorbents. The only compound less efficiently (83%) retained by this sorbent was cyclamate. Bakerbond SDB-1 shows unusual selectivity towards aspartame and alitame. Recoveries of these two sweeteners were very low (26 and 42% respectively). It was also found that aspartame and alitame can be selectively separated from the mixture of sweeteners using formic acid - triethylamine buffer at pH 3.5.

Autorzy

• Agata Zygler link otwiera się w nowej karcie ,
• prof. dr hab. inż. Andrzej Wasik link otwiera się w nowej karcie ,
• prof. dr hab. inż. Jacek Namieśnik link otwiera się w nowej karcie