Repozytorium publikacji - Politechnika Gdańska

Freeze-dried cells of Meiothermus ruber catalyses cleavage of o-nitrophenyl-b-D-galactopiranoside (oNPb-gal) and conversion of lactose into glucose and galactose. The permeabilization with 2%toluene,20%ethanol and 20%acetone increased enzymatic activity from 74.87 U/g of lyophilized cells up to 129.44,114.38 and 90.19 U/g,respectively. Ethanol was an effective permeabilizing agent and its efficiency was dependent on the concentration, the incubation time and incubation temperature. The Km values for the untreated and permeabilized cells were 2.94 mM and 2.26 mM but Vmax values were 122 mmol/min and 193mmol/min, respectively. The optimum pH for the b-galactosidase activity in the untreated and permeabilized cells were 6.5 and optimum of temperatures 65C.The stabilit yof enzymatic activity in M.ruber cells incubated for 1 h at pH 6.5 was almost unchanged at temperatures below 65C.

Autorzy

• prof. dr hab. Józef Synowiecki link otwiera się w nowej karcie ,
• dr inż. Izabela Sinkiewicz link otwiera się w nowej karcie ,
• Agata Zakrzewska,
• Katarzyna Grubiak link otwiera się w nowej karcie ,
• mgr inż. Olga Pietrow-Tobiszewska link otwiera się w nowej karcie