Loop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six to eight distinct sequences on the target DNA. The amplification is performed by DNA polymerase, which has strand-displacing activity. The whole reaction occurs in a single tube and is divided into two steps: non-cyclic and cyclic. The final products are artificial stem loop DNA structures flanking the target sequences.
Autorzy
Informacje dodatkowe
- Kategoria
- Publikacja monograficzna
- Typ
- rozdział, artykuł w książce - dziele zbiorowym /podręczniku w języku o zasięgu międzynarodowym
- Język
- angielski
- Rok wydania
- 2013
