Repozytorium publikacji - Politechnika Gdańska

DNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several promoter regions in two setups implementing different references: mock-digested and treated with a restriction enzyme that has no recognition sites within examined amplicons. Fragmentation of reference templates allowed removing the overestimation effect, thereby improving measurement accuracy.

Autorzy

• Magdalena Krygier,
• dr inż. Justyna Podolak-Popinigis link otwiera się w nowej karcie ,
• Prof. dr hab. Janusz Limon,
• prof. dr hab. inż. Paweł Sachadyn link otwiera się w nowej karcie ,
• Anna Stanisławska-Sachadyn