Repozytorium publikacji - Politechnika Gdańska

Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purifcation prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharifcation conditions and identify the process parameters that should be applied to improve the saccharifcation efciency of lignocellulosic biomass, defned as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at diferent stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharifcation enzymatic cocktail were identifed and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.

Autorzy

• dr inż. Karolina Kucharska link otwiera się w nowej karcie ,
• dr inż. Edyta Słupek link otwiera się w nowej karcie ,
• dr hab. inż. Hubert Cieśliński link otwiera się w nowej karcie ,
• prof. dr hab. inż Marian Kamiński