The point spread function (PSF) of imaging systems plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view. In confocal macroscopy, the related artifacts are even stronger, as the field of view is much larger. Because the related PSFs are strongly spatially variant, it is essential to be able to model them with few parameters. The field aberrations in fluorescence macroscopy were observed to be symmetrical and to increase with the distance from the center of the field of view. In this paper we propose an experiment and an optimization method for assessing the center of the field of view. The obtained results constitute an important step towards reducing the number of parameters in macroscopy PSF models.
Autorzy
- dr inż. Anna Węsierska link otwiera się w nowej karcie ,
- Hugues Talbot,
- Jean-Christophe Pesquet
Informacje dodatkowe
- DOI
- Cyfrowy identyfikator dokumentu elektronicznego link otwiera się w nowej karcie 10.1109/isbi.2018.8363622
- Kategoria
- Aktywność konferencyjna
- Typ
- materiały konferencyjne indeksowane w Web of Science
- Język
- angielski
- Rok wydania
- 2018
Źródło danych: MOSTWiedzy.pl - publikacja "Spatially variant PSF modeling in confocal macroscopy" link otwiera się w nowej karcie
