Repozytorium publikacji - Politechnika Gdańska

Gender differences in the burden of cardiovascular disease (CVD) have been observed worldwide. In this study, plasmatic levels of trimethylamine (TMA) and blood oxidative biomarkers have been evaluated in 358 men (89 controls and 269 CVD patients) and 189 women (64 control and 125 CVD patients). The fluorescence technique was applied to determine erythrocyte membrane fluidity using 1,6-diphenyl-1,3,5-hexatriene (DPH) and Laurdan, while lipid hydroperoxides were assessed by diphenyl − 1-pyrenylphosphine (DPPP). Results show that levels of plasmatic TMA were higher in healthy men with respect to healthy women (p = 0.0001). Significantly lower TMA was observed in male CVD patients (0.609 ± 0.104 μM) compared to healthy male controls (0.680 ± 0.118 μ M) ( p < 0.001), while higher levels of TMA were measured in female CVD patients (0.595 ± 0.115 μ M) with respect to female controls (0.529 ± .073 μ M) ( p < 0.001). DPPP was significantly higher in healthy control men than in women (p < 0.001). Male CVD patients displayed a lower value of DPPP (2777 ± 1924) compared to healthy controls (5528 ± 2222) ( p < 0.001), while no significant changes were measured in females with or without CVD (p > 0.05). Membrane fluidity was significantly higher ( p < 0.001) in the hydrophobic bilayer only in control male subjects. In conclusion, gender differences were observed in blood oxidative biomarkers, and DPPP value might be suggested as a biomarker predictive of CVD only in men.

Autorzy

• Laura Bordoni,
• Donatella Fedeli,
• Marco Piangerelli,
• Iwona Pelikant-Malecka,
• Adrianna Radulska,
• Joanna J. Samulak,
• Angelika K. Sawicka,
• Lukasz Lewicki,
• prof. dr hab. lek. Leszek Kalinowski link otwiera się w nowej karcie ,
• Robert A. Olek,
• Rosita Gabbianelli