Repozytorium publikacji - Politechnika Gdańska

Pre-treatment of diamond surface in low-temperature plasma for oxygenation and in acids for carboxylation was hypothesized to promote the branching density of the hyperbranched glycidol polymer. This was expected to increase the homogeneity of the branching level and suppress interactions with proteins. As a result, composite nanodiamonds with reduced hydrodynamic diameters that are maintained in physiological environments were anticipated. Surfaces of 140-nm-sized nanodiamonds were functionalized with oxygen and carboxyl groups for grafting of hyperbranched dendritic polyglycerol via anionic ring-opening polymerization of glycidol. The modification was verified with Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. Dynamic light scattering investigated colloidal stability in pH-diverse (2–12) solutions, concentrated phosphate-buffered saline, and cell culture media. Thermogravimetric analysis of nanodiamonds-protein incubations examined non-specific binding. Fluorescence emission was tested across pH conditions. Molecular dynamics simulations modeled interparticle interactions in ionic solutions. The hyperbranched polyglycerol grafting increased colloidal stability of nanodiamonds across diverse pH, high ionic media like 10 × concentrated phosphate-buffered saline, and physiological media like serum and cell culture medium. The hyperbranched polyglycerol suppressed non-specific protein adsorption while maintaining intensive fluorescence of nanodiamonds regardless of pH. Molecular modelling indicated reduced interparticle interactions in ionic solutions correlating with the improved colloidal stability.

Autorzy

• dr inż. Maciej Głowacki,
• Dr Paweł Niedziałkowski,
• dr hab. inż. Jacek Ryl link otwiera się w nowej karcie ,
• dr inż. Marta Prześniak-Welenc link otwiera się w nowej karcie ,
• dr hab. inż. Mirosław Sawczak,
• Klaudia Prusik link otwiera się w nowej karcie ,
• dr inż. Mateusz Ficek link otwiera się w nowej karcie ,
• Monika Janik,
• mgr inż. Krzysztof Pyrchla link otwiera się w nowej karcie ,
• Michał Olewniczak link otwiera się w nowej karcie ,
• dr Krzysztof Bojarski link otwiera się w nowej karcie ,
• prof. dr hab. inż. Jacek Czub link otwiera się w nowej karcie ,
• dr hab. inż. Robert Bogdanowicz link otwiera się w nowej karcie