Repozytorium publikacji - Politechnika Gdańska

This study evaluates the potential of rapid microbiological methods, traditionally used for food and environmental samples, to assess human milk (HM) quality in Human Milk Banks. Several methods, including a bioluminescence, the Micro Biological Survey (MBS) colorimetric method, triphenyltetrazolium chloride (TTC) reductase test, and flow cytometry (BacSomatic™️), were compared to the PN-EN ISO 4833–1:2013-12 reference method. Flow cytometry showed a strong correlation with the reference method for estimating bacterial counts in HM, indicating its potential as a fast alternative. Despite its speed − providing results in 10 min without pre-processing − flow cytometry showed some limitations suggesting it may not yet be suitable as a standalone tool. Other methods, such as TTC reduction test and bioluminescence, showed weaker correlations, indicating that external factors influence their accuracy, while flow cytometry is promising, further optimization is required before it can replace conventional methods.

Autorzy

• mgr inż. Katarzyna Mazur link otwiera się w nowej karcie ,
• dr hab. inż. Edyta Malinowska-Pańczyk link otwiera się w nowej karcie ,
• Izabela Drążkowska,
• dr inż. Magdalena Chmara link otwiera się w nowej karcie