Publications Repository - Gdańsk University of Technology

Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding b-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory prax

Authors

• Natalia Kobylak link open in new tab ,
• dr n. med. Barbara Bykowska,
• Ewelina Kurzyk link open in new tab ,
• prof. dr hab. Roman Nowicki,
• dr hab. inż. Anna Brillowska-Dąbrowska link open in new tab