Repozytorium publikacji - Politechnika Gdańska

Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding b-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory prax

Autorzy

• Natalia Kobylak link otwiera się w nowej karcie ,
• dr n. med. Barbara Bykowska,
• Ewelina Kurzyk link otwiera się w nowej karcie ,
• prof. dr hab. Roman Nowicki,
• dr hab. inż. Anna Brillowska-Dąbrowska link otwiera się w nowej karcie