Fungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based on cytochrome c oxidase subunit 1 and β-tubulin genes. The assays resulted in 2–10 restriction patterns, depending on the gene amplified and restriction enzyme applied. Pooled analysis of the patterns allowed to propose an algorithm, that can be successfully used for an accurate species-specific identification of 21 species of the Scopulariopsis-like fungi.
Authors
- Milena Kordalewska,
- Jolanta Kalita,
- Zofia Bakuła,
- dr hab. inż. Anna Brillowska-Dąbrowska link open in new tab ,
- Tomasz Jagielski
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1093/mmy/myy106
- Category
- Publikacja w czasopiśmie
- Type
- artykuł w czasopiśmie wyróżnionym w JCR
- Language
- angielski
- Publication year
- 2019
