Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Å resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.
Authors
- Begoña Heras,
- Melissa A. Edeling,
- Karl A. Byriel,
- Alun Jones,
- prof. dr Satish Raina link open in new tab ,
- Jennifer L. Martin
Additional information
- DOI
- Digital Object Identifier link open in new tab 10.1016/s0969-2126(03)00005-4
- Category
- Publikacja w czasopiśmie
- Type
- artykuły w czasopismach
- Language
- angielski
- Publication year
- 2003
