Repozytorium publikacji - Politechnika Gdańska

Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Å resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.

Autorzy

• Begoña Heras,
• Melissa A. Edeling,
• Karl A. Byriel,
• Alun Jones,
• prof. dr Satish Raina link otwiera się w nowej karcie ,
• Jennifer L. Martin