Repozytorium publikacji - Politechnika Gdańska

Derivatization is a frequently used sample prepara-tion procedure applicable to the enhancement of analyte de-tection sensitivity. Amino acids mostly require derivatization prior to electrophoretic or chromatographic analysis, especial-ly if spectrophotometric detection is used. This study presents an on-line preconcentration technique for derivatized amino acids. The sensitivity of the method was improved by the utilization of the proposed acid-induced pH-mediated stacking mechanism. The method is demonstrated by preconcentration of amino acids labeled with 2,4-dinitrofluorobenzene. Use of optimized conditions for a large sample volume injection (40 s, 13.8 kPa) followed by electrokinetic injection of 0.1 M HCl (20 s, 10 kV) gave a 20- to 30-fold enhancement of sensitivity. The significance of the sweeping mechanism and pseudo-isotachophoresis for the on-line sample focusing and the influence of parameters on the preconcentration pro-cess were discussed. The applicability of the elaborated meth-od was demons ated using human urine samples.

Autorzy

• Szymon Dziomba,
• dr hab. inż. Adrian Bekasiewicz link otwiera się w nowej karcie ,
• Adam Prahl,
• Tomasz Baczek,
• Piotr Kowalski