2023 - Publications Repository - Gdańsk University of Technology

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Publications from the year 2023

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Impact of climate change on groundwater recharge in shallow young glacial aquifers in northern Poland
- Anna Gumuła-Kawęcka
- Beata Jaworska-Szulc
- Adam Szymkiewicz
- Wioletta Gorczewska-Langner
- Rafael Angulo-Jaramillo
- Jirka Simunek
2023 SCIENCE OF THE TOTAL ENVIRONMENT
We investigated the influence of climate change in the period 1951–2020 on shallow aquifers in the Brda and Wda outwash plains (Pomeranian Region, Northern Poland). There was a significant temperature rise (0.3 °C/10 years), which accelerated after 1980 (0.66 °C/10 years). Precipitation became increasingly irregular – extremely rainy years occurred right after or before extremely dry years, and intensive rainfall events became more frequent after 2000. The groundwater level decreased over the last 20 years, even though the average annual precipitation was higher than in the previous 50 years. We carried out numerical simulations of water flow in representative soil profiles for the years 1970–2020 using the HYDRUS-1D model, developed and calibrated during our earlier work at an experimental site in the Brda outwash plain (Gumuła-Kawęcka et al., 2022). We used a relationship between the water head and flux at the bottom of the soil profiles (the third-type boundary condition) to reproduce groundwater table fluctuations caused by recharge variability in time. The calculated daily recharge showed a decreasing linear trend for the last 20 years (0.05–0.06 mm d−1/10 years), and dropping trends in water table level and soil water content in the entire profile of vadose zone. Field tracer experiments were performed to estimate impact of extremely rain events on water flux in vadose zone. The results suggest that tracer travel times are strongly determined by water content in the unsaturated zone which is determined by precipitation amount in span of weeks, rather than extremely high precipitation events.
Impact of Climate Change on Water Sources and River‐Floodplain Mixing in the Natural Wetland Floodplain of Biebrza River
- Tomasz Edward Berezowski
- Daniel Partington
2023 Full text WATER RESOURCES RESEARCH
The origins of river and floodplain waters (groundwater, rainfall, and snowmelt) and their extent during overbank flow events strongly impact ecological processes such as denitrification and vegetation development. However, the long-term sensitivity of floodplain water signatures to climate change remains elusive. We examined how the integrated hydrological model HydroGeoSphere and the Hydraulic Mixing-Cell method could help us understand the long-term impact of climate change on water signatures and their spatial distribution in the protected Biebrza River Catchment in northeastern Poland. Our model relied on 20th century Reanalysis Data from 1881 to 2015 and an ensemble of EURO-CORDEX simulations for representative concentration pathways (RCP) 2.6, 4.5, and 8.5 from 2006 to 2099. The historical component of the simulations was subjected to extensive multiple-variable validation from 1881 to 2019. The results show that the extents of water sources were rather stable in the floodplain in the 1881–2015 period. The projected future impacts were variable with each analyzed RCP, but in all cases, different significant trends were present for the spatial distribution of water sources and for the river-floodplain mixing. However, the total volume of water from different sources was less sensitive to climate change than the dominant sources and spatial distribution of water. The simulation results highlight the impact of climate change on the extent of water sources in temperate zone wetlands with significant implications for ecological processes and management. These results also underscore the urgent need to leverage such modeling studies to inform protective and preservation strategies of floodplain wetlands.
Impact of laser beam welding on mechanical behaviour of 2.25Cr–1Mo (P22) steel
- Sachin Sirohi
- Shailesh M. Pandey
- Vinita Tiwari
- Dhowmya Bhatt
- Dariusz Fydrych
- Chandan Pandey
2023 INTERNATIONAL JOURNAL OF PRESSURE VESSELS AND PIPING
The use of welding processes in the manufacturing and repair of structures intended for the energy industry plays a key role in the guarantee of a continuous supply of fossil fuels, which is the basic condition for ensuring energy security. A square butt joint of 10 mm thick plate of 2.25Cr–1Mo (P22) steel was fabricated by autogenous laser beam welding process and then post-weld heat treatment (PWHT) for two sets of process parameters (760 °C for 90 min–T1 and 730 °C for 180 min–T2). The joints were subjected to detailed metallographic investigations and hardness measurements, static tensile tests and impact toughness tests. The joint in as-welded state is characterized by significant heterogeneity of structural morphology and mechanical properties, while PWHT significantly improves uniformity in microstructure and mechanical properties along weldments. PWHT in particular causes tempering of the bainite and promotes the formation of carbide precipitates (M7C3, M23C6, and Mo2C) of globular and spherical shape along boundaries and bainite blocks. Laser welding caused a drastic increase in the weld metal hardness (388 HV) and coarse prior austenite grain bainite region (390 HV) compared to the hardness of base metal (BM) (below 200 HV). Both of the applied PWHT variants reduced the hardness of these areas of welded joints to the BM level. During the tensile tests, all the joints were fractured in BM. In the case of AW joints the tensile strength was higher than BM and it was 625 ± 10 MPa and after both PWHT, tensile strength was decreased by about 100 MPa. Due to over tempering of bainite in BM the elongation of the joints was several percentage points lower (27 ± 3 for AW joints) than the value for BM (35% ± 2), but slightly higher in the case of joints after PWHT (31 and 33 ± 1). Impact energy of the weld metal (259 ± 8 J) was lower than P22 base metal (320 ± 8 J), but PWHT causes a significant increase in impact energy of the weld metal (291 ± 6 J and 306 ± 10 J for T1 and T2, respectively). The analysis of these results shows that the proposed laser welding procedure with a high temperature gradient and PWHT enables the fabrication of P22 steel welded joints that meet the quality criteria.
Impact of rotor geometry optimization on the off-design ORC turbine performance
- Łukasz Witanowski
- Piotr Klonowicz
- Piotr Lampart
- Piotr Klimaszewski
- Tomasz Suchocki
- Łukasz Jędrzejewski
- Dawid Zaniewski
- Paweł Ziółkowski
2023 ENERGY
The paper describes the method of CFD based Nelder-Mead optimization of a 10 kW single-stage axial turbine operating in an ORC system working on R7100. The total-to-static isentropic efficiency is defined as an objective function. Multi-point linear regression is carried out to determine the significance of the objective function arguments and to pick up the set of particular variables and characteristic quantities (e.g. flow angles) which contribute most to improving the objective function value. This approach enables us to considerably reduce the number of optimized parameters and computational time needed for the optimization process. Variable operating parameters of ORC power systems become an everyday occurrence, which makes the analysis of ORC turbines under variable operating parameters a critical part of the design. Therefore, the paper presents the influence of a single-criterion optimization of the rotor geometry on the performance of the ORC turbine in a wide range of operating conditions. The results of optimization indicate that new geometry provides an approximately 5.5 pp. (percentage point) increase in total-to-static efficiency for the design point, and even larger improvements reaching 7 pp. For higher turbine loads, whereas for lower loads the efficiency improvements are at the level of 1.5–4 pp.
Impact of soluble organic matter and particulate organic matter on anammox system: Performance, microbial community and N2O production
- Yingying He
- Hongyu Mao
- Jacek Mąkinia
- Jakub Drewnowski
- Bing Wu
- Jun Xu
- Li Xie
- Xi Lu
2023 JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
In this study, the effects of soluble readily biodegradable COD (sCOD) and particulate slowly biodegradable COD (pCOD) on anammox process were investigated. The results of the longterm experiment indicated that a low sCOD/N ratio of 0.5 could accelerate the anammox and denitrification activity, to reach as high as 84.9% ±2.8% TN removal efficiency. Partial denitrification-anammox (PDN/anammox) and denitrification were proposed as the major pathways for nitrogen removal, accounting for 91.3% and 8.7% of the TN removal, respec- tively. Anammox bacteria could remain active with high abundance of anammox genes to maintain its dominance. Candidatus Kuenenia and Thauera were the predominant genera in the presence of organic matter. Compared with sCOD, batch experiments showed that the introduction of pCOD had a negative effect on nitrogen removal. The contribution of denitrification to nitrogen removal decreased from approximately 14% to 3% with increasing percentage of pCOD. In addition, the analysis result of the process data using an optimized ASM1 model indicated that high percentage of pCOD resulted in serious N 2 O emission (the peak value up to 0.25 mg N/L), which was likely due to limited mass diffusion and insufficient available carbon sources for denitrification. However, a high sCOD/N ratio was beneficial for alleviating N 2 O accumulation.
Impact of steel grade on a ship colliding with an offshore wind turbine monopile supporting structure
- Karol Niklas
- Alicja Bera
- Yordan Garbatov
2023 Full text OCEAN ENGINEERING
Offshore wind power is experiencing rapid development around the world. The number of installations and their size is growing. Thousands of monopile support structures with diameters of 10 m and weights of 2000 tons will be installed in the coming years. Offshore wind power installations, often located close to the shore and shipping routes, pose a new challenge to ship safety. The study analyses the damages from an accidental collision between a 6500-tonne displacement Supply Offshore Vessel and the monopile support structure with a 10 m diameter and a 15 MW wind turbine. The head-on collision cases are performed according to the DNV-ST-0126, showing the consequences for the ship and the wind turbine support structure. In addition, simulation cases with an offset between the ship’s plane of symmetry and the monopile axis showed a new form of damage to the ship’s plating. During a head-on sliding collision, extensive plate tearing can occur due to its cutting at the deck line and concertina tearing under the pressure of the monopile. As a result, the hull may open over a large area, including the threat of unsealing the ship’s collision bulkhead. The S355 grade steel can significantly reduce collision damage by up to 50% (from 20.6 m2 to 10.5 m2 for the case studied here).
Impact of strontium non-stoichiometry of SrxTi0.3Fe0.7O3-δ on structural, electrical, and electrochemical properties for potential oxygen electrode of intermediate temperature solid oxide cells
- Aleksander Mroziński
- Sebastian Molin
- Patryk Błaszczak
- Tadeusz Miruszewski
- Karolina Górnicka
- Jakub Karczewski
- Piotr Jasiński
2023 Full text INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
This work presents the results of a comprehensive study on the impact of the A-site non-stoichiometry of SrxTi0.3Fe0.7O3-δ (x = 0.90, 0.95, 1.00, 1.05) ceramics on their physicochemical properties. The materials were fabricated by the conventional solid-state reaction method and their structure was determined by X-ray diffractometry, X-ray photoelectron spectroscopy and electron microscopy. Their sintering and thermal expansion properties were then evaluated. The electrical properties of the materials were determined by electrical conductivity and electrical relaxation measurements (on bulk materials) and by electrochemical impedance spectroscopy (EIS) studies of symmetrical, CGO-electrolyte based, porous electrodes. Finally, fuel cell tests with the non-stoichiometric electrodes were evaluated. To elucidate the electrochemical reaction pathways for oxygen reduction/evolution reaction, EIS measurements were carried out in different pO2 and were analysed via the distribution of relaxation times method. The results showed a dependence of materials’ properties on the A-site non-stoichiometry, which can be used to fine-tune their properties, e.g. increase the surface exchange rate or decrease the thermal expansion coefficient.
Impact of temperature on optical sensing in biology based on investigation of SARS‐CoV ‐2
- Małgorzata Szczerska
- Paweł Wityk
- Paulina Listewnik
2023 Full text Journal of Biophotonics
In this paper, we present an investigation of the influence of the temperature on the sensing of biological samples. We used biofunctionalized microsphere-based fiber-optic sensor to detect immunoglobulin G attached to the sensor head at temperatures relevant in biological research: 5°C, 25°C, and 55°C. The construction of the sensor allowed us to perform measurements in the small amount of solution. The results of our experiment confirm substantial changes in the measured reflected optical power, indicating the need to control the temperature during such measurements. The sensitivity of the sensor used in this research is 8.82 nW/°C. Coefficient R was also calculated and it equals 0.998, which shows good fit between theoretical linear fit and obtained measured data.
Impact of thermal backfill parameters on current-carrying capacity of power cables installed in the ground
- Seweryn Szultka
- Stanisław Czapp
- Adam Tomaszewski
2023 Full text Bulletin of the Polish Academy of Sciences-Technical Sciences
Proper design of power installations with the participation of power cables buried in homogeneous and thermally well-conductive ground does not constitute a major problem. The situation changes when the ground is non-homogeneous and thermally low-conductive. In such a situation, a thermal backfill near the cables is commonly used. The optimization of thermal backfill parameters to achieve the highest possible current-carrying capacity is insufficiently described in the standards. Therefore, numerical calculations based on computational fluid dynamics could prove helpful for designers of power cable lines. This paper studies the influence of dimensions and thermal resistivity of the thermal backfill and thermal resistivity of the native soil on the current-carrying capacity of power cables buried in the ground. Numerical calculations were performed with ANSYS Fluent. As a result of the research, proposals were made on how to determine the current-carrying capacity depending on the dimensions and thermal properties of the backfill. A proprietary mathematical function is presented which makes it possible to calculate the cable current-carrying capacity correction factor when the backfill is used. The research is expected to fill the gap in the current state of knowledge included in the provisions of standards.
Implementacja aktów prawa w metodyce badań emisyjności akustycznej w transporcie szynowym
- Roksana Licow
- Natalia Karkosińska-Brzozowska
- Anna Świerkoska
- Wiktor Żółkoś
- Przemysław Barszcz
- Julia Szulta
- Kamil Łukaszewicz
- Izabela Muzyka
- Aleks Gołąbek
- Magdalena Jóźwik
- Daria Kassin
- Michał Polakowski
- Mateusz Racki
- Maciej Wolski
- Bartosz Nowak
2023
Przedmiotem artykułu jest przedstawienie zakresu działań wraz ze wstępnymi wnioskami, które dotyczą realizowanego projektu Plutonium Supporting Student Research Teams pn. Badania emisyjności akustycznej w transporcie szynowym realizowanym przez Koło Naukowe Inżynierii Drogowej i Kolejowej Politechniki Gdańskiej. Celem projektu jest ocena emisyjności akustycznej typu nawierzchni z typem pojazdu szynowego oraz ocena komfortu podróżnych w transporcie kolejowym i tramwajowym w kontekście hałasu. Realizacja badań została zaplanowana dwutorowo:  pomiary zewnątrz pojazdu w kontekście zależności poziomu dźwięku w odniesieniu do określonych typów nawierzchni,  pomiary wewnątrz pojazdów w kontekście wpływu hałasu na komfort podróżnych. Zespół projektowy został podzielony na dwie grupy badawcze, jedna z grup działa w zakresie hałasu emitowanego przez pociągi, druga zaś hałasu emitowanego przez tramwaje. W artykule zawarto analizę literatury w tym norm oraz aktów prawnych dotyczących hałasu emitowanego we wewnątrz i na zewnątrz pojazdów szynowych. Analizę przeprowadzano dla pojazdów kolejowych oraz tramwajów. Na podstawie wniosków opracowano szereg metodyk prowadzenia badań akustycznych celem implementacji ich w realizacji eksperymentów na rzeczywistych obiektach podczas ich eksploatacji. Opracowana metodyka badań może być podstawą do zmian prawnych w sposobach i metodach oceny hałasu na zewnątrz i przede wszystkim wewnątrz pojazdów szynowych np. z uwzględnieniem różnych typów wagonów.
Implementation of an Adaptive Method for Changing the Frequency Division of the Counter Clock Signal in a Frequency-to-Code Converter
- Dariusz Świsulski
- Piotr Warda
2023 Full text ENERGIES
Processing physical quantities into an indirect signal is a standard method of transferring information about the measured quantity to the master system, which analyzes the data obtained from the acquisition system. The intermediate signal is very often the voltage, but another transmission medium can be the frequency of the output signal of the “physical quantity-to-frequency” converter. The article presents the implementation of the adaptive method of selecting the clock signal frequency of the counter working in the converter. The issue of selecting the clock signal frequency for the required processing range of the transducer is discussed in detail. The application of the method using the STM32L476RG microcontroller is presented. The principle of checking the processing range of the developed transducer model is discussed. The algorithms of transducer operation in basic and adaptive modes of measuring the period of the variable frequency signal are proposed. The results of operation, in both modes, of the transducer model of frequency processing are presented, along with the metrological analysis of the results. The influence of selected approximations used to reconstruct the measured quantity on the final presentation of the measurement result is discussed.
Implementation of IMS/NGN Transport Stratum Based on the SDN Concept
- Sylwester Kaczmarek
- Maciej Sac
- Kamil Bachorski
2023 Full text SENSORS
The paper presents the development and verification of software and a testbed aiming to demonstrate the ability of two telecommunication network concepts—Next Generation Network (NGN) and Software-Defined Networking (SDN)—to cooperate. The proposed architecture includes components of the IP Multimedia Subsystem (IMS) in its service stratum and of the SDN (controller and programmable switches) in its transport stratum, providing flexible transport resource control and management via open interfaces. One important feature of the presented solution is the inclusion of ITU-T standards for NGN networks, which are not considered in other related works. The paper includes details regarding the hardware and software architecture of the proposed solution as well as results of the performed functional tests, which confirm its proper operation.
IMPLEMENTING THE QUALITY ASSURANCE SYSTEM AND ASSURING HEALTH SAFETY IN THE LIGHT OF INCREASING BREAD QUALITY
- Radosław Drozd
- Radosław Wolniak
2023 Full text Zeszyty Naukowe. Organizacja i Zarządzanie / Politechnika Śląska
The article presents rules, requirements, and norms for quality management and health safety assurance systems among small and medium bakeries. This group of businesses does not have the obligation of implementing or certifying specific systems. They are, however, implemented in order to increase the quality of the bread or reach a better market position. Also, the results of a 2022 study of 53 bakeries’ implementation of individual quality management systems are presented in the paper. The study shows an important role of the implementation of coordinated systems in the increase of quality of bread. Theoretical considerations and the results of a questionnaire study allowed the author to discuss and explain why neither the TQM system nor the BRC standards were implemented in small- and medium- sized bakeries. The results of research on the implementation of quality management systems and ensuring the health safety of bread confirm the legitimacy of using integrated quality systems. They will have a fundamental impact in the long-term perspective of innovative management of bakery managers and owners.
Improved methods for stator end winding leakage inductance calculation
- Grzegorz Kostro
- Michał Michna
- Filip Kutt
- Roland Ryndzionek
2023 Full text COMPEL-THE INTERNATIONAL JOURNAL FOR COMPUTATION AND MATHEMATICS IN ELECTRICAL AND ELECTRONIC ENGINEERING
Calculating the stator end-winding leakage inductance, taking into account the rotor, is difficult due to the irregular shape of the end-winding. The end-winding leakage may distribute at the end of the active part and the fringing flux of the air gap. The fringing flux belongs to the main flux but goes into the end-winding region. Then, not all the magnetic flux occurring in the end region is the end-winding leakage flux. The purpose of this paper was to find a method to accurately separate the leakage from the total flux, taking into account the rotor.
Improved-Efficacy EM-Driven Optimization of Antenna Structures Using Adaptive Design Specifications and Variable-Resolution Models
- Sławomir Kozieł
- Anna Pietrenko-Dąbrowska
2023 IEEE TRANSACTIONS ON ANTENNAS AND PROPAGATION
Optimization-driven parameter tuning is an essential step in the design of antenna systems. Although in many cases it is still conducted through parametric studies, rigorous numerical methods become a necessity if truly optimum designs are sought for, and the problem intricacies (number of variables, multiple goals, constraints) make the interactive approaches insufficient. The two practical considerations of electromagnetic (EM)-driven optimization are reliability and computational cost. Repetitive EM simulations may incur unmanageable expenses, whereas the lack of a decent starting point or objective function multimodality may prevent the numerical procedures (especially the local ones) from identifying satisfactory designs. In pursuit of reliability improvements, a design specification adjustment procedure has been recently proposed that improves the immunity of local search procedures to poor starting points. Therein, the objective function is modified by relocating the design goals (e.g., center frequencies) closer to the actual operating parameters of the antenna at the current design, to make them attainable through local search. The goals are then gradually adjusted and converge to the original targets towards then end of the optimization process. In this paper, we introduce an algorithm for reliable and low-cost antenna tuning that capitalizes on the specification management scheme while embedding it in variable-resolution optimization framework. In our approach, the EM model fidelity is adaptively adjusted based on the misalignment between the actual and target operating conditions, as well as the convergence status of the algorithm. By initiating the search process from the lowest-fidelity model (gradually dialed up to the highest-fidelity one), considerable computational savings of almost sixty percent can be achieved, with respect to the single-fidelity procedure. The speedup is possible without compromising reliability of the optimization process, as demonstrated using three examples of microstrip antennas, designed under different and challenging scenarios.
Improvement of speech intelligibility in the presence of noise interference using the Lombard effect and an automatic noise interference profiling based on deep learning
- Krzysztof Kąkol
2023 Full text
The Lombard effect is a phenomenon that results in speech intelligibility improvement when applied to noise. There are many distinctive features of Lombard speech that were recalled in this dissertation. This work proposes the creation of a system capable of improving speech quality and intelligibility in real-time measured by objective metrics and subjective tests. This system consists of three main components: speech type detection, noise profiling, and an adaptive strategy of selection the modification. The role of the first component is to detect the Lombard speech in the input signal to avoid unnecessary speech modifications when the speech is naturally Lombard in its character. The second module is noise profiling, as the type of noise strongly impacts the selection of the best modification. The last part of the system is the adaptive modification selection component. The selection is made based on the speech signal features, resulting in the most considerable speech quality improvement, measured with objective metrics. To solve the problem posed, machine learning was used in this dissertation – especially deep learning with convolutional neural networks and typical multilayer networks. It was proven that it is possible to create an adaptive system that would improve speech quality in the presence of noise in real-time or near real-time.
Improving carbon dioxide capture in aqueous ammonia solutions by fine SiO2 particles
- Donata Konopacka-Łyskawa
- Temesgen Amibo
- Adam Dobrzyniewski
- Marcin Łapiński
2023 Full text Chemical and Process Engineering : New Frontiers
Ammonia solutions are considered to be effective solvents for carbon dioxide absorption. Despite the numerous advantages of these solvents, their high volatility is a significant technical and economic problem. Therefore, in this work, silica particles were used as additives to improve CO2 absorption and inhibit NH3 desorption. SiO2 microparticles and colloidal SiO2 particles in the concentration range of 0-0.15 wt.% were used in this study. The most favorable mass transport for CO2 absorption was at the concentration of colloidal particles of 0.05 wt.%. Under these conditions, the enhancement in the number of moles of absorbed CO2 was above 30%. However, in solvents containing 0.01 wt.% SiO2 microparticles, the increase in CO2 absorption was about 20%. At the same time, the addition of SiO2 particles significantly reduced the escape of ammonia from the solution. The best improvement was obtained when colloidal SiO2 particles were added, and then NH3 escape was decreased by about 60%. This unfavorable phenomenon was also inhibited in ammonia solutions containing SiO2 microparticles at a concentration of 0.01 wt.%.
In situ transformation boosts the pseudocapacitance of CuNi-MOF via cooperative orientational and electronic governing
- Xianyu Chu
- Fanling Meng
- Wei Zhang
- Lihua Zhang
- Sebastian Molin
- Piotr Jasiński
- Weitao Zheng
2023 Full text Materials Research Letters
The disordered arrangement and thereof inferior conductivity of 2D MOF sheets seriously hinder their practical application. Herein, we propose in situ transformation strategy to architect vertically oriented bimetallic CuNi-MOF as a self-supporting electrode, leading to a decuple high specific capacitance of 1262 C g-1 in comparison with the pristine Ni-MOF powder of 114 C g-1 at 2 A g-1. DFT calculations reveal that introduction of Cu can modulate the electronic structure of metal centers in the Ni-MOF sheets and optimize electrical conductivity. Our strategy is promising to maximize the utilization of MOF superiorities for optimizing their electrochemical performance.
In vitro and in silico assessment of anti-inflammatory activity of cocoa powders
- Jelena S. Katanić Stanković
- Vanja Todorović
- Jelena Dorović Jovanović
- Zoran Marković
- Sanja Krstić
- Nevena Dabetić
- Sladana Sobajić
- Agnieszka Bartoszek-Pączkowska
- Zoran Maksimovic
- Rudolf Bauer
2023
Plants are considered the major sources of biologically active compounds, which provide unlimited opportunities for their use either as medical treatments or as novel drug formulations. Cocoa powder is frequently used in nutrition and is known to have many benefits thanks to its wide range of biological activities. The presented study was focused on th evaluation of the anti-inflammatory potential of extracts obtained from cocoa powder. In vitro assays were employed to evaluate the level of inhibition of cyclooxygenases-1 and -2 activities (COX-1 and COX-2) by tested extracts. Molecular docking was used for in silico prediction of cyclooxygenase isoforms inhibition by selected cocoa powder constituents. The results showed that all tested extracts exerted much higher potential in inhibiting COX-2 activity and may be considered in use as selective inhibitors of COX-2 enzyme. On the other hand, in silico study shows quercetin and clovamide as the compounds with the highest potential to inhibit both COX-1 and COX-2.
In Vitro and In Vivo Imaging-Based Evaluation of Doxorubicin Anticancer Treatment in Combination with the Herbal Medicine Black Cohosh
- Agata Płoska
- Marcin Woźniak
- Jamila Hedhli
- Christian J. Konopka
- Antonios Skondras
- Sarah Matatov
- Andrew Stawarz
- Sarah Schuh
- Andrzej Czerwinski
- Lawrence W. Dobrucki
- Leszek Kalinowski
- Iwona T. Dobrucki
2023 Full text INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
As a substitution for hormone replacement therapy, many breast cancer patients use black cohosh (BC) extracts in combination with doxorubicin (DOX)-based chemotherapy. In this study, we evaluated the viability and survival of BC- and DOX-treated MCF-7 cells. A preclinical model of MCF-7 xenografts was used to determine the influence of BC and DOX administration on tumor growth and metabolism. The number of apoptotic cells after incubation with both DOX and BC was significantly increased (~100%) compared to the control. Treatment with DOX altered the potential of MCF-7 cells to form colonies; however, coincubation with BC did not affect this process. In vivo, PET-CT imaging showed that combined treatment of DOX and BC induced a significant reduction in both metabolic activity (29%) and angiogenesis (32%). Both DOX and BC treatments inhibited tumor growth by 20% and 12%, respectively, and combined by 57%, vs. control. We successfully demonstrated that BC increases cytotoxic effects of DOX, resulting in a significant reduction in tumor size. Further studies regarding drug transport and tumor growth biomarkers are necessary to establish the underlying mechanism and potential clinical use of BC in breast cancer patients.