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Publications from the year 2024
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Tailoring Defects in B, N-Codoped Carbon Nanowalls for Direct Electrochemical Oxidation of Glyphosate and its Metabolites
- Mattia Pierpaoli
- Paweł Jakóbczyk
- Mateusz Ficek
- Bartłomiej Dec
- Jacek Ryl
- Bogdan Rutkowski
- Aneta Lewkowicz
- Robert Bogdanowicz
Tailoring the defects in graphene and its related carbon allotropes has great potential to exploit their enhanced electrochemical properties for energy applications, environmental remediation, and sensing. Vertical graphene, also known as carbon nanowalls (CNWs), exhibits a large surface area, enhanced charge transfer capability, and high defect density, making it suitable for a wide range of emerging applications. However, precise control and tuning of the defect size, position, and density remain challenging; moreover, due to their characteristic labyrinthine morphology, conventional characterization techniques and widely accepted quality indicators fail or need to be reformulated. This study primarily focuses on examining the impact of boron heterodoping and argon plasma treatment on CNW structures, uncovering complex interplays between specific defect-induced three-dimensional nanostructures and electrochemical performance. Moreover, the study introduces the use of defect-rich CNWs as a label-free electrode for directly oxidizing glyphosate (GLY), a common herbicide, and its metabolites (sarcosine and aminomethylphosphonic acid) for the first time. Crucially, we discovered that the presence of specific boron bonds (BC and BN), coupled with the absence of Lewis-base functional groups such as pyridinic-N, is essential for the oxidation of these analytes. Notably, the D+D* second-order combinational Raman modes at ≈2570 cm–1 emerged as a reliable indicator of the analytes’ affinity. Contrary to expectations, the electrochemically active surface area and the presence of oxygen-containing functional groups played a secondary role. Argon-plasma post-treatment was found to adversely affect both the morphology and surface chemistry of CNWs, leading to an increase in sp3-hybridized carbon, the introduction of oxygen, and alterations in the types of nitrogen functional groups. Simulations support that certain defects are functional for GLY rather than AMPA. Sarcosine oxidation is the least affected by defect type.
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Tailoring Physicochemical Properties of V2O5 Nanostructures: Influence of Solvent Type in Sol-Gel Synthesis
- Klaudia Prusik
- Daniel Jaworski
- Justyna Gumieniak
- Agnieszka Kramek
- Kamila Sadowska
- Marta Prześniak-Welenc
The influence of different solvents, including aqueous and nonaqueous types, on the physicochemical properties of V2O5 nanostructures was thoroughly investigated. Various characterization techniques, such as XRD, XPS, FTIR, Raman spectroscopy, UV-vis DRS, SEM, TEM, and BET, were employed to analyze the obtained materials. Additionally, the adsorption properties of the synthesized V2O5 nanostructures for methylene blue were examined, and kinetic parameters of adsorption were calculated. The results demonstrate that the morphology of the obtained crystals can be finely controlled by manipulating water concentration in the solution, showcasing its profound impact on both the structural characteristics and adsorption properties of the nanostructures. Furthermore, the structural changes of the resulting V2O5 material induced by solvents show strong impacts on its photocatalytic properties, making it a promising photocatalyst.
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Tailoring the Transport Layer Interface for Relative Indoor and Outdoor Photovoltaic Performance
- Chia-Feng Li
- Shih-Han Cheng
- Hou-Chin Cha
- Ssu-Yung Chung
- Damian Głowienka
- Chih-Min Chuang
- Yu-Ching Huang
The ability to achieve notable indoor power conversion efficiency (PCE) makes organic photovoltaics (OPV) a potential technology for indoor applications. Currently, ongoing research in indoor OPVs focuses on improving both their indoor PCE and their stability. However, little attention has been given to investigating the fabrication yield of indoor OPVs, a pivotal determinant of their potential commercial viability. In this study, we discovered that despite assessing the PCE of OPVs under indoor and solar illumination conditions using the same devices, the fabrication yields under these distinct light sources vary significantly. Employing diverse analytical measurements, we elucidated the underlying mechanisms contributing to this variance. Our findings suggest that disparities in fabrication yield resulted from the interfacial interactions between the hole transport layer (HTL) and the active layer. Particularly, the interfacial behavior between these layers plays a decisive role in achieving elevated fabrication yields in indoor OPVs. Furthermore, we demonstrate the function of a combination of two HTLs (TAPC/MoO3), which not only enhances the indoor PCE of OPVs but also substantially improves the fabrication yield of indoor OPVs. Our study offers insights and critical guidance for the advancement of indoor OPVs with high fabrication yields.
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Tailor-Made Polysaccharides for Biomedical Applications
- Mohsen Khodadadiyazdi
- Farzad Seidi
- Aleksander Hejna
- Payam Zarrintaj
- Navid Rabiee
- Justyna Kucińska-Lipka
- Mohammad Saeb
- Sidi A. Bencherif
Polysaccharides (PSAs) are carbohydrate-based macromolecules widely used in the biomedical field, either in their pure form or in blends/nanocomposites with other materials. The relationship between structure, properties, and functions has inspired scientists to design multifunctional PSAs for various biomedical applications by incorporating unique molecular structures and targeted bulk properties. Multiple strategies, such as conjugation, grafting, cross-linking, and functionalization, have been explored to control their mechanical properties, electrical conductivity, hydrophilicity, degradability, rheological features, and stimuli-responsiveness. For instance, custom-made PSAs are known for their worldwide biomedical applications in tissue engineering, drug/gene delivery, and regenerative medicine. Furthermore, the remarkable advancements in supramolecular engineering and chemistry have paved the way for mission-oriented biomaterial synthesis and the fabrication of customized biomaterials. These materials can synergistically combine the benefits of biology and chemistry to tackle important biomedical questions. Herein, we categorize and summarize PSAs based on their synthesis methods, and explore the main strategies used to customize their chemical structures. We then highlight various properties of PSAs using practical examples. Lastly, we thoroughly describe the biomedical applications of tailor-made PSAs, along with their current existing challenges and potential future directions.
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Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment
- Aleksandra Kuplińska
- Kamila Rząd
- Joanna Stefaniak-Skorupa
- Katarzyna Kozłowska-Tylingo
- Marek Wojciechowski
- Sławomir Milewski
- Iwona Gabriel
Fungal infections are a serious threat to public health as they are becoming increasingly frequent. A major problem stems also from a rising fungal resistance to currently available antifungal therapies, therefore novel molecular targets are highly desirable. Exploration of enzymes participating in the biosynthesis pathways of essential amino acids such as L-methionine (L-Met) may provide new insights into pharmaceutical development. The MET15 gene from Candida albicans, encoding O- acetyl-L-homoserine sulfhydrylase (Met15p), an enzyme catalyzing the second step in that pathway, was cloned and expressed in two versions: as N and C-terminal oligo-His-tagged fusion proteins. The recombinant enzymes revealed appropriate activity, and catalyzed conversion of O-acetyl-L-homoserine and a sulfide ion to produce L-homocysteine. A new RP-HPLC-DAD method, using the enzymatic reaction product pre-column derivatization with 5,5’-dithio-bis-(2-nitrobenzoic acid) was developed and used by us to determine Met15p activity. Newly synthesized compounds as well as two commercially available exhibited a Met15p inhibitory effect which was related to antifungal activity. Fungal cells’ sensitivity to inhibitors depending on the presence or absence of L-Met in the medium clearly indicated Met15p targeting. Moreover, the synergistic effect of the first methionine biosynthetic enzyme affecting inhibitor and Met15p inhibitors indicate that methionine biosynthesis pathway enzymes are promising molecular targets.
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Targeting shelterin proteins for cancer therapy.
- Wioletta Brankiewicz-Kopcinska
- Anoop Kallingal
- Radosław Krzemieniecki
- Maciej Bagiński
As a global health challenge, cancer prompts continuous exploration for innovative therapies that are also based on new targets. One promising avenue is targeting the shelterin protein complex, a safeguard for telomeres crucial in preventing DNA damage. The role of shelterin in modulating ataxia- telangiectasia mutated (ATM) and ataxia-telangiectasia and Rad3-related (ATR) kinases, key players in the DNA damage response (DDR), establishes its significance in cancer cells. Disrupting these defence mechanisms of shelterins, especially in cancer cells, renders telomeres vulnerable, potentially leading to genomic instability and hindering cancer cell survival. In this review, we outline recent approaches exploring shelterins as potential anticancer targets, highlighting the prospect of developing selective molecules to exploit telomere vulnerabilities toward new innovative cancer treatments.
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Targeting yeast topoisomerase II by imidazo and triazoloacridinone derivatives resulting in their antifungal activity
- Kamila Rząd
- Iwona Gabriel
- Ewa Paluszkiewicz
- Aleksandra Kuplińska
- Mateusz Olszewski
- Agnieszka Chylewska
- Aleksandra M. Dąbrowska
- Katarzyna Kozłowska-Tylingo
Fungal pathogens are considered as serious factors for deadly diseases and are a case of medical concern. Invasive fungal infections also complicate the clinical course of COVID-19, leading to a significant increase in mortality. Furthermore, fungal strains' multidrug resistance has increased the demand for antifungals with a different mechanism of action. The present study aimed to identify antifungal compounds targeting yeast topoisomerase II (yTOPOII) derived from well-known human topoisomerase II (hTOPOII) poisons C-1305 and C-1311. Two sets of derivatives: triazoloacridinones (IKE1-8) and imidazoacridinones (IKE9-14) were synthetized and evaluated with a specific emphasis on the molecular mechanism of action. Our results indicated that their effectiveness as enzyme inhibitors was not solely due to intercalation ability but also as a result of influence on catalytic activity by the formation of covalent complexes between plasmid DNA and yTOPOII. Lysine conjunction increased the strength of the compound's interaction with DNA and improved penetration into the fungal cells. Triazoloacridinone derivatives in contrast to starting compound C-1305 exhibited moderate antifungal activity and at least twice lower cytotoxicity. Importantly, compounds (IKE5-8) were not substrates for multidrug ABC transporters whereas a derivative conjugated with lysine (IKE7), showed the ability to overcome C. glabrata fluconazole-resistance (MIC 32–64 µg mL−1).
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Task-recency bias strikes back: Adapting covariances in Exemplar-Free Class Incremental Learning
- Grzegorz Rypeść
- Sebastian Cygert
- Tomasz Trzciński
- Bartłomiej Twardowski
Exemplar-Free Class Incremental Learning (EFCIL) tackles the problem of training a model on a sequence of tasks without access to past data. Existing state-of-the-art methods represent classes as Gaussian distributions in the feature extractor's latent space, enabling Bayes classification or training the classifier by replaying pseudo features. However, we identify two critical issues that compromise their efficacy when the feature extractor is updated on incremental tasks. First, they do not consider that classes' covariance matrices change and must be adapted after each task. Second, they are susceptible to a task-recency bias caused by dimensionality collapse occurring during training. In this work, we propose AdaGauss - a novel method that adapts covariance matrices from task to task and mitigates the task-recency bias owing to the additional anti-collapse loss function. AdaGauss yields state-of-the-art results on popular EFCIL benchmarks and datasets when training from scratch or starting from a pre-trained backbone.
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Teaching High–performance Computing Systems – A Case Study with Parallel Programming APIs: MPI, OpenMP and CUDA
- Paweł Czarnul
- Mariusz Matuszek
- Adam Krzywaniak
High performance computing (HPC) education has become essential in recent years, especially that parallel computing on high performance computing systems enables modern machine learning models to grow in scale. This significant increase in the computational power of modern supercomputers relies on a large number of cores in modern CPUs and GPUs. As a consequence, parallel program development based on parallel thinking has become a necessity to fully utilize modern HPC systems' computational power. Therefore, teaching HPC has become essential in developing skills required by the industry. In this paper we share our experience of conducting a dedicated HPC course, provide a brief description of the course content, and propose a way to conduct HPC laboratory classes, in which a single task is implemented using several APIs, i.e., MPI, OpenMP, CUDA, hybrid MPI+Pthreads, and MPI+OpenMP. Based on the actual task of verifying Goldbach's conjecture for a given range of numbers, we present and analyze the performance evaluation of students' solutions and code speed-ups for MPI and OpenMP. Additionally, we evaluate students' subjective assessment of ease of use of particular APIs along with the lengths of codes, and students' performance over recent years.
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Teaching of Robot Arm Programming - Tasks with Increasing Difficulty
- Marek Chodnicki
- Alina Guzik
- Marek Galewski
- Wiktor Sieklicki
- Michał Mazur
The article describes education techniques using robotics. For example, educational exercises on programming the Nachi MZ04 robotic arm are presented. The exercises use a wooden railroad toy model and elements printed in additive manufacturing technology. The exercises have increasing difficulty and can be used to educate young people in high school and specialized higher engineering studies (both bachelor's and master's).
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Team research project – evolution from faculty activity to university study standard
- Mariusz Kaczmarek
- Krzysztof Nowicki
The article will describe the idea of a university-wide team student project as a tool of modern academic teaching in the era of widespread use of artificial intelligence tools. The problem of contemporary teaching is the emerging tools for automatic content generation, including didactic and scientific content. The question arises how to verify students' qualifications and learning outcomes. The solution may be students' group work on an interdisciplinary problem and a comprehensive approach to solving it. The article will present the current experience of the Faculty of Electronics, Telecommunications and Computer Science in the implementation of student group and research projects and the assumptions of changes in the study programs at the Gdańsk University of Technology (GUT) introducing a university-wide student team research project during master's studies.
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Techno-economic analysis of a hybrid energy system for electrification using an off-grid solar/biogas/battery system employing HOMER: A case study in Vietnam
- Van Bac Nguyen
- Prabhakar Sharma
- Bhaskor Jyoti Bora
- Thi Minh Tu Bui
- Cristina Efremov
- Minh Ho Tran
- Jerzy Kowalski
- Sameh M. Osman
- Dao Nam Cao
- Van Huong Dong
The electrification of off-grid /island villages is a critical step towards improving the techno-economic circumstances of rural regions and the overall general growth of the country. However, consistent supply from a single source is not possible in these areas. Thus, a hybrid renewable energy system performs better in these conditions. The research challenge now is to identify the optimal combinations of HRES from the available resources in a specific village site that can supply the power demand sustainably and to determine whether this is a cost-effective option. The present work is an endeavour to develop a sustainable and dynamic energy demandsupply model using HOMER Pro energy software in a specified off-grid rural site in Vietnam. The research presents four unique configurations of a combined energy system for Vietnam’s island settlements, incorporating biomass-based biogas facilities, photovoltaic panels, lithium-ion batteries, and converters. Homer Pro was used for optimization and design, focusing on key performance measures such as cost of energy, net present cost, initial cost, operating cost, renewable fraction, and carbon emissions. The best HES system layout includes a 100- kW biomass-based generator, 2.62 kW photovoltaic installation, 10 lithium-ion batteries, and a 6.31 kW converter, producing 100 % renewable energy. The system’s low cost of energy ($0.48), and net present cost ($25,730.89) make it an economically viable alternative, while its low CO2 emissions demonstrate its commitment to reducing greenhouse gas emissions.
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Technological solutions to landfill management: Towards recovery of biomethane and carbon neutrality
- Hussein Al-Hazmi
- Gamal K. Hassan
- Tonni Agustiono Kurniawan
- Bogna Śniatała
- Tomy M. Joseph
- Joanna Majtacz
- Grzegorz Piechota
- Xiang Li
- Fatma A. El-Gohary
- Mohammad Saeb
- Jacek Mąkinia
Inadequate landfill management poses risks to the environment and human health, necessitating action. Poorly designed and operated landfills release harmful gases, contaminate water, and deplete resources. Aligning landfill management with the Sustainable Development Goals (SDGs) reveals its crucial role in achieving various targets. Urgent transformation of landfill practices is necessary to address challenges like climate change, carbon neutrality, food security, and resource recovery. The scientific community recognizes landfill management's impact on climate change, evidenced by in over 191 published articles (1998–2023). This article presents emerging solutions for sustainable landfill management, including physico-chemical, oxidation, and biological treatments. Each technology is evaluated for practical applications. The article emphasizes landfill management's global significance in pursuing carbon neutrality, prioritizing resource recovery over end-of-pipe treatments. It is important to note that minimizing water, chemical, and energy inputs in nutrient recovery is crucial for achieving carbon neutrality by 2050. Water reuse, energy recovery, and material selection during manufacturing are vital. The potential of water technologies for recovering macro-nutrients from landfill leachate is explored, considering feasibility factors. Integrated waste management approaches, such as recycling and composting, reduce waste and minimize environmental impact. It is conclusively evident that the water technologies not only facilitate the purification of leachate but also enable the recovery of valuable substances such as ammonium, heavy metals, nutrients, and salts. This recovery process holds economic benefits, while the conversion of CH4 and hydrogen into bioenergy and power generation through microbial fuel cells further enhances its potential. Future research should focus on sustainable and cost-effective treatment technologies for landfill leachate. Improving landfill management can mitigate the adverse environmental and health effects of inadequate waste disposal.
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Temperature Effects on Weigh-in-Motion Accuracy Using Steering Axle Load Spectrum
- Dawid Ryś
- Przemysław Więckowski
Weigh-in-motion systems (WIM) serve as valuable sources of traffic load data. The determination of axle load spectra using WIM data is pivotal for calculating load equivalency factors and predicting pavement distresses through M-EPDG. Among various factors affecting WIM accuracy, temperature changes stand out as particularly influential. This paper examines the influence of temperature on WIM-collected data, utilizing the steering axle load spectrum. Data was gathered from 77 WIM stations situated on Poland's national roads and motorways. Observations reveal that temperature fluctuations introduce biases into the axle load spectrum, significantly impacting several key statistics, including Truck Factors (TF) and number of Equivalent Standard Axle Loads (ESAL) or the percentage of overloaded vehicles. Notably, a shift in air temperature from -10°C to +30°C leads to axle load spectrum biases ranging from 5% to 35%. The technology of axle load sensors plays a crucial role in this phenomenon. The analysis indicates that the uncertainty of truck factors and the subsequent calculation of ESAL’s using the fourth power equation can increase by up to 1.5 times when the axle load spectrum bias reaches 20%.
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Temperature Measurements at Tyre Tread Rubber on Sandpaper Oscillatory Sliding Contacts Using Acicular Grindable Thermocouples
- Oleksii Nosko
- Yurii Tsybrii
- Pablo Guillermo Torrelio Arias
- Adolfo Senatore
The tribological performance of tyre–road contacts depends crucially on the contact temperature. This study investigates the reliability and accuracy of acicular grindable thermocouples possessing an original needle-shaped wearable part as applied to measuring temperature at the oscillatory sliding contact between a rubber tyre tread sample and a sandpaper. A linear oscillatory tribometer is used to imitate the sliding phase of tyre–road contact under mild friction conditions. It is shown that the acicular grindable thermocouple measurements are generally test–retest repeatable. Moreover, the thermocouple signal becomes more stable with increasing contact pressure. Compared to the conventional thermocouple technique, the acicular grindable thermocouple overestimates temperature at the rubber friction surface by about 23% due to involvements of its wearable part in friction with the sandpaper. The findings suggest an expansion of the acicular grindable thermocouple technique to full-scale experiments with tyres on the road.
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Teoria grafów wczoraj i dziś
- Marek Kubale
W pracy naszkicowano kamienie milowe teorii grafów poczynając od pierwszego artykułu Eulera na temat mostów w Królewcu z połowy 18. wieku. Następnie opisano słynny problem 4 barw i jego wariacje. Pracę kończy charakterystyka najnowszych wyzwań teorii grafów.
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Terahertz Dual-Band Metamaterial Biosensor for Cervical-Cancer Diagnostics
- Musa N. Hamza
- Mohammad Alibakhshikenari
- Bal Virdee
- Muhamad A. Hamad
- Salahuddin Khan
- Sławomir Kozieł
- Ernesto Limiti
This study highlights the potential of employing terahertz metamaterial structures as dual-band biosensors for the early detection of cancerous biological tissue. The fundamental principle leveraged here is the alteration of the effective dielectric constant of biological tissue by cancerous cells. The change in the dielectric constant, in turn, induces a shift in the resonance frequency of the metamaterial sensor. One notable advantage of the terahertz metamaterial sensor is its relatively compact size compared to other sensor types, as its dimensions are independent of the wavelength. This property translates into a requirement for a much smaller biopsy sample, facilitating less invasive testing procedures. Beyond the size advantage, the proposed biosensor demonstrates efficacy in detecting abnormalities within biological tissue.
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Testing Topological Conjugacy of Time Series
- Justyna Signerska-Rynkowska
- Paweł Dłotko
- Michał Lipiński
This paper considers a problem of testing, from a finite sample, a topological conjugacy of two trajectories coming from dynamical systems (X, f ) and (Y, g). More precisely, given x1, . . . , xn \subset X and y1, . . . , yn \subset Y such that xi+1 = f (xi) and yi+1 = g(yi) as well as h : X \rightarrow Y, we deliver a number of tests to check if f and g are topologically conjugated via h. The values of the tests are close to zero for systems conjugate by h and large for systems that are not. Convergence of the test values, in the case when the sample size goes to infinity, is established. We provide a number of numerical examples indicating scalability and robustness of the presented methods. In addition, we show howthe presented method gives rise to a test of sufficient embedding dimension, mentioned in Takens' embedding theorem. Our methods also apply to the situation when we are given two observables of deterministic processes, of a form of one or higher dimensional time series. In this case, their similarity can be assessed by comparing the dynamics of their Takens' reconstructions. Finally, we include a proof-of-concept study using the presented methods to search for an approximation of the homeomorphism conjugating given systems.
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Tetracycline degradation for wastewater treatment based on ozone nanobubbles advanced oxidation processes (AOPs) – Focus on nanobubbles formation, degradation kinetics, mechanism and effects of water composition
- Priya Koundle
- Neelkanth Nirmalkar
- Malwina Momotko
- Sławomir Makowiec
- Grzegorz Boczkaj
Presence of pharmaceuticals, especially antibiotics, in industrial and domestic effluents causes serious damage to the environment. Classic wastewater treatment processes, in particular conventional biological treatment methods, are not sufficient to rapidly eliminate antibiotics. Typically, Advanced Oxidation Processes (AOPs) based on activation of hydrogen peroxide, ozone or persulfate for formation of particular type of radical species or singlet oxygen are used. A one of cutting-edge technologies to increase effectiveness of AOPs based on ozone are nanobubbles based processes. Thus, this paper focuses on utilization of ozone in the form of nanobubbles for degradation of tetracycline (TC). The effects of several reaction parameters, such as antibiotic concentration, ozone intake, pH, presence of salts, were investigated. This study revealed that the presence of ozone nanobubbles had a substantial positive impact on the degradation of TC. This improvement may be attributed to the enhanced mass transfer and the production of reactive radicals that occur during the collapse of the nanobubbles. Identification of Reactive Oxygen Species (ROS) revealed a significant contribution of hydroxyl radicals in the degradation of the antibiotic. AOP based on O3 nanobubbles generated mostly hydroxyl (•OH) and superoxide anion (O2•–) radicals providing 100 % degradation of 100 mg/L TC within 20 min at 8 mg/L ozone concentration. Based on identified by LC-MS intermediates a detailed degradation mechanism has been described. Degradation of TC and intermediates transformations included methylation, hydroxylation, ring-opening steps as well as cleavage of C-N bonds. This research introduces a novel technique combining nanobubbles with advanced oxidation processes (AOPs), which is anticipated to provide enhanced efficiency and environmental sustainability.
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The Application of a Mobile Unmanned Device for Monitoring Water and Sediment Pollution in the Port of Gdynia
- Aleksandra Bojke
- Katarzyna Galer-Tatarowicz
- Agnieszka Flasińska
- Andrzej Chybicki
- Zbigniew Łubniewski
- Jadwiga Kargol
- Dominika Ostrowska
- Agnieszka Cichowska
Pollution in the Port of Gdynia can encompass various types of substances and contaminants that affect the quality of water and sediment in this region. Ships entering and leaving the port can release pollutants such as oil, fuel, waste, and chemicals into the water. Controlling and monitoring these pollutants is a crucial part of environmental stewardship. In recent years, uncrewed units have been increasingly in use for in situ water and sediment sampling. Boat-based water sampling crews face significant safety risks at sea. Eliminating the need for a boat-based sampling crew, uncrewed units allow sampling from locations that are difficult to access by traditional sampling methods. To validate the HydroDron-1 method, water samples and bottom sediments were collected from five basins in the Port of Gdynia using the HydroDron-1 method and accredited sampling methods. The values of measured parameters were similar for both methods. Using the HydroDron-1 method at the Port of Gdynia has the potential to improve safety while reducing sampling costs and increasing data collection. The project was implemented as part of the POLNOR 2019 Call program, announced by the National Center for Research and Development (NCRD): “Marine port surveillance and observation system using mobile unmanned research units” NOR/POLNOR/MPSS/0037/2019.