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Publikacje z roku 2017
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A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond
- Dawid Nidzworski
- Siuzdak Katarzyna
- Paweł Niedziałkowski
- Robert Bogdanowicz
- Michał Sobaszek
- Jacek Ryl
- Paulina Weiher
- Mirosław Sawczak
- Elżbieta Wnuk
- William A. Goddard III
- Andrés Jaramillo-botero
- Tadeusz Ossowski
According to the World Health Organization (WHO), almost 2 billion people each year are infected worldwide with flu-like pathogens including influenza. This is a contagious disease caused by viruses belonging to the family Orthomyxoviridae. Employee absenteeism caused by flu infection costs hundreds of millions of dollars every year. To successfully treat influenza virus infections, detection of the virus during the initial development phase of the infection is critical, when tens to hundreds of virus-associated molecules are present in the patient’s pharynx. In this study, we describe a novel universal diamond biosensor, which enables the specific detection of the virus at ultralow concentrations, even before any clinical symptoms arise. A diamond electrode is surface-functionalized with polyclonal anti-M1 antibodies, which then serve to identify the universal biomarker for the influenza virus, M1 protein. The absorption of the M1 protein onto anti-M1 sites of the electrode change its electrochemical impedance spectra. We achieved a limit of detection of 1 fg/ml in saliva buffer for the M1 biomarker, which corresponds to 5–10 viruses per sample in 5 minutes. Furthermore, the universality of the assay was confirmed by analyzing different strains of influenza A virus.
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A repeated game formulation of network embedded coding for multicast resilience in extreme conditions
- Christian Esposito
- Aniello Castiglione
- Francesco Palmieri
- Florin Pop
- Jacek Rak
Computer networks and data sharing applications are vital for our current society and fundamental for any available ICT solution, so that networking is considered as one of the key critical infrastructures and its correct behavior should be always enforced, even in case of disasters or severe execution conditions. Resilience is a strongly demanding nonfunctional requirement for current computer networks, and one of the key factors to provide it is represented by loss tolerance. From wired to wireless networks, exchanged packets can be lost due to routing anomalies or temporary malfunctioning at the networking hardware or software. The experienced loss pattern can be exacerbated by severe weather conditions in wireless environments and/or due to effects of a disaster. Proper recovery schemes must be put in place in order to provide loss tolerance and move a step forward realizing disaster-resilient networks. This work contributes to such an objective by proposing a loss-tolerant scheme based on forward error correction and properly implementing it in a distributed manner, by having multiple nodes generating coded information to be used for the reconstruction of the lost data without retransmissions. The problem of placing and tuning such coding nodes within a communication infrastructure has been tackled according to a repeated game theory formulation, and a preliminary evaluation demonstrates the achievable improvements in the delivery success rate with limited costs.
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A review of phosphorus recovery methods at various steps of wastewater treatment and sewage sludge management. The concept of “no solid waste generation” and analytical methods
- Bartłomiej Cieślik
- Piotr Konieczka
Phosphorus deposits around the world are rapidly depleting, therefore phosphorus recovery methods are gaining more and more interest both in science and industry. This article presents the main methods of phosphorus recovery from sewage sludge. The described approaches are divided in two groups: phosphorus recovery from sewage sludge and leachate, and recovery of phosphorus from sewage sludge ashes. The latter seems to have more advantages connected with both ecological and economical aspects. The need for development of “no solid waste generation” strategy is becoming more and more urgent. The concept of comprehensive management of all solid residues after what is currently considered the most ecological process of sewage sludge incineration connected with phosphorus recovery based on acidic extraction, is described in the article. Solid residues after phosphorus recovery from sewage sludge ashes by means of acidic extraction can be stabilized with solid residues after sewage sludge incineration exhaust gas treatment. Such an approach may enable production of phosphoric raw material together with stabilized construction material. Advantages and disadvantages of the discussed approaches are given. An analysis of the composition of ashes produced in different sewage sludge treatment plants indicates that the proposed technology could be successfully applied in most of such units, especially because the concentrations of elements such as K, Mg, Na, P are sufficiently high, respectively 1.5 - 12.1 g/kg; 9.9 - 14.9 g/kg; 3.6 - 13,3 g/kg and 7,4 - 99,0 g/kg. However, a phosphorus recovery method should be developed separately for each treatment plant. Only then all comprehensive management methods will be ecologically and economically justified. Analytical methods which could be of use at every step of designing a proper phosphorus recovery process are described.
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A role of the heat and work uncompensatedtransformations in the balance of entropy and theturbomachinery efficiency
- Tomasz Kowalczyk
- Paweł Ziółkowski
- Daniel Sławiński
- Janusz Badur
- Małgorzata Cisak
In the paper we have presented a procedure of estimating a role of the uncompensated transformation of heat and work in ow of viscous and heat conducting uid. The procedure was introduced by Professor Romuald Puzyrewski and is essential in local, in time and space, balance of entropy. Furthermore this unique approach leads to redenition the eciency of uid-ow machinery in three-dimensional modeling using CFD (Computational Fluid Dynamics) methods. The study has shown that usage of the polytropic eciency, instead of the isentropic eciency, is more comfortable and seems to be more natural in CFD approach. Helpful is also correlation between those two eciency denitions with usage of proposed Pu number.
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A scale with ECG measurements capability for home cardiac monitoring
- Mariusz Kaczmarek
- Adam Bujnowski
- Kamil Osiński
- Jerzy Wtorek
Presented device -a scale developed for enabling extra measurements, in addition to body weight and composition, the electrical activity of the heart (electrocardiogram - ECG) and the level of oxygen saturation in the blood SaO2. Electrical activity is recorded in the form of six leads, socalled - limb leads. The device allows measurement of all standard limb leads and without the need for additional procedures. ECG, although the measurement is performed in an unconventional standard, and the results should be treated as if they were made using a standard ECG monitor.
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A Semiautomatic Experience-Based Tool for Solving Product Innovation Problem
- Mohammad Waris
- Cesar Sanín
- Edward Szczerbicki
In this paper we present the idea of Smart Innovation Engineering (SIE) System and its implementation methodology. The SIE system is semi-automatic system that helps in carrying the process of product innovation. It collects the experiential knowledge from the formal decisional events. This experiential knowledge is collected from the group of similar products having some common functions and features. The SIE system behaves like a group of experts in its domain as it collects, captures and stores the experiential knowledge from similar products as well as reuses this experiential knowledge that ultimately enhances the innovation process of manufactured goods. Moreover, with SIE in hand entrepreneurs and manufacturing organizations will be able to take proper, enhanced decisions and most importantly at appropriate time. This expertise is ever increasing as every decision taken is stored in SIE system in the form of set of experience that can be used in future for similar queries. Implementation of the SIE System using Set of Experience Knowledge Structure (SOE) and Decisional DNA (DDNA) for case study suggests that the SIE system is capable of capturing and reusing the innovation related experiences of the manufactured products. The case study confirmed that the SIE system can be beneficial for entrepreneurs and manufacturing organizations for efficient decision-making in the product innovation process.
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A SHORT REVIEW OF BLOOD FLOW MODELLING METHODS: FROM MACRO- TO MICROSCALES
- Katarzyna Kaczorowska-Ditrich
- Krzysztof Tesch
The aim of this paper it to review various scale approaches to the blood flow modelling. Blood motion may be described by three types of mathematical models according to the observed scales or resolutions, namely microscopic, mesoscopic and macroscopic descriptions. The above approaches are discussed together with their advantages and disadvantages. Several results of mesoscopic simulations are presented with particular attention paid to mesoscale semi-continuum models suitable for real-time blood flow visualisation.
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A simplified energy dissipation based model of heat transfer for subcooled flow boiling
- Jarosław Mikielewicz
- Dariusz Mikielewicz
In the paper a model is presented based on energetic considerations for subcooled flow boiling heat transfer. The model is the extension of authors own model developed earlier for saturated flow boiling and condensation. In the former version of the model we used the heat transfer coefficient for the liquid single-phase as a reference level, due to the lack of the appropriate model for heat transfer coefficient for the subcooled flow boiling. That issue was a fundamental weakness of the that approach. The purpose of present investigation is to fulfil this drawback. Now the reference heat transfer coefficient for the saturated flow boiling in terms of the value taking into account the subcooled flow conditions. The wall heat flux is based on partitioning and constitutes of two principal components, namely the convective heat flux and partial evaporation heat flux of the liquid replacing the detached bubble. Both terms are accordingly modeled. The convective heat flux is regarding vapour bubbles travelling longitudinally and the liquid moving radially – liquid pumping. The results of calculations have been compared with some experimental data from literature showing a good consistency.
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A spatio-temporal approach to intersectoral labour and wage mobility
- Karol Flisikowski
The article presents the spatio-temporal approach for intersectoral labor and wage mobility. Analyses of interindustry mobility were performed with the use of general entropy mobility indices (GEMM). Spatio- temporal approach was obtained thanks to the separate measurement of spatial autocorrelation and regression for each set of sectoral wage and employment structure and was conducted in each year of the research period separately. Calculations of economic distance were based on the level of GDP, whereas in spatial regression data of previously calculated mobility indices were used. Because of the availability of homogeneous, highly aggregated sectoral data only for the period 1994-2011, the analyses were performed for 20 selected OECD countries. The use of spatial error model (SEM) and spatial lag model (SLM) improved explanatory abilities of the analysis and revealed that the higher level of interindustry wage mobility is accompanied by increased movement of labor force across sectors.
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A Spatio-temporal Approach to Intersectoral Labour and Wage Mobility
- Karol Flisikowski
The article presents the spatio-temporal approach for intersectoral labor and wage mobility. Analyses of interindustry mobility were performed with the use of general entropy mobility indices (GEMM). Spatio-temporal approach was obtained thanks to the separate measurement of spatial autocorrelation and regression for each set of sectoral wage and employment structure and was conducted in each year of the research period separately. Calculations of economic distance were based on the level of GDP, whereas in spatial regression data of previously calculated mobility indices were used. Because of the availability of homogeneous, highly aggregated sectoral data only for the period 1994-2011, the analyses were performed for 20 selected OECD countries. The use of spatial error model (SEM) and spatial lag model (SLM) improved explanatory abilities of the analysis and revealed that the higher level of interindustry wage mobility is accompanied by increased movement of labor force across sectors.
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A study of the photoelectron spectra of α-tetrahydrofurfuryl alcohol over the outer valence energy region (9–25 eV)
- Marcin Dampc
- Brygida Mielewska
- Michele Siggel-King
- George C. King
- Mariusz Zubek
Photoelectron spectra of α-tetrahydrofurfuryl alcohol (THFA) molecules, the threshold spectrum and spectra for constant photoelectron energies, have been measured over the photon energy range 9–25 eV. Analysis of the photoelectron spectra has allowed overlapping photoionization bands to be separated, tentatively assigned and their vertical photoionization energies to be determined. These energies are compared with existing theoretical calculations. The photoelectron spectra indicate the predominance of one conformer in the population of gas-phase THFA.
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A Survey on Identifying and Addressing Business Analysis Problems
- Aleksander Jarzębowicz
- Piotr Marciniak
Despite the growing body of knowledge on requirements engineering and business analysis, these areas of software project are still considered problematic. The paper focuses on problems reported by business analysts and on applicability of available business analysis techniques as solutions to such problems. A unified set of techniques was developed on the basis of 3 industrial standards associated with IIBA, REQB and IREB certification schemes. A group of 8 business analysts was surveyed to list problems they encounter in their work and to assess their frequency. Selected problems were further analyzed and most suitable techniques were proposed to address them. These proposals were validated through follow-up discussions with business analysts. The main results of research reported in this paper are: the comparative analysis of techniques included in IIBA, REQB and IREB standards and the list of problems reported by practitioners associated with techniques suggested as effective solutions.
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A tetragonal polymorph of SrMn2P2 made under high pressure – theory and experiment in harmony
- Weiwei Xie
- Michał Jerzy Winiarski
- Tomasz Klimczuk
- R.j. Cava
Following the predictions of total energy calculations, a tetragonal SrMn2P2 phase is proposed and successfully formed under high pressure. At ambient pressure, SrMn2P2 adopts the primitive trigonal La2O3 structure type (space group P[3 with combining macron]m1). However, the results of total energy calculations indicate that SrMn2P2 should be more stable in the tetragonal ThCr2Si2 structure type (space group I4/mmm) than in its known trigonal structure, thus motivating our synthetic experiments. Guided by these calculations, a new tetragonal polymorph of SrMn2P2 was found under the relatively mild conditions of 5 GPa applied pressure at a temperature of 900 °C through the transformation of the ambient pressure trigonal form. The new polymorph has the body centered tetragonal ThCr2Si2 structure type, as predicted. The electronic structure calculations indicate the likelihood of antiferromagnetic, semiconducting properties for the high pressure SrMn2P2 phase.
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A Thermographic Measurement Approach to Assess Supercapacitor Electrical Performances
- Stanisław Galla
This paper describes a proposal for the qualitative assessment of condition of supercapacitors based on the conducted thermographic measurements. The presented measurement stand was accompanied by the concept of methodology of performing tests. Necessary conditions, which were needed to minimize the influence of disturbing factors on the performance of thermal imaging measurements, were also indicated. Mentioned factors resulted from both: the hardware limitations and from the necessity to prepare samples. The algorithm that was used to determine the basic parameters for assessment has been presented. The article suggests to use additional factors that may facilitate the analysis of obtained results. Measuring the usefulness of the proposed methodology was tested on commercial samples of supercapacitors. All of the tests were taken in conjunction with the classical methods based on capacitance (C) and equivalent series resistance (ESR) measurements, which were also presented in the paper. Selected results presenting the observed changes occurring in both: basic parameters of supercapacitors and accompanying fluctuations of thermal fields, along with analysis, were shown. The observed limitations of the proposed assessment method and the suggestions for its development were also described.
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A two dimensional approach for modelling of pennate muscle behaviour
- Wiktoria Wojnicz
- Bartlomiej Zagrodny
- Michal Ludwicki
- Jan Awrejcewicz
- Edmund Wittbrodt
The purpose of this study was to elaborate a two-dimensional approach for unipennate and bipennate striated skeletal muscle modelling. Behavior of chosen flat pennate muscle is modelled as a rheological system composed of serially linked passive and active fragments having different mechanical properties. Each fragment is composed of three elements: mass element, elastic element and viscous element. Each active fragment furthermore contains the contractile element. Proposed approach takes into consideration that muscle force depends on a planar arrangement of muscle fibers. Paper presents results of numerical simulations, conclusions deduced on the base of these results and a concept of experimental verification of proposed models.
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
- Agata Wołoszyn
- Roman Kotłowski
ABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
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A white phosphor based on oxyfluoride nano-glass-ceramics co-doped with Eu3+ and Tb3+: Energy transfer study
- Andrzej Kłonkowski
- Wiesław Wiczk
- Jacek Ryl
- Karol Szczodrowski
- Dorota Wileńska
Strontium fluoride nanocrystals doped separately with Ln3+ = Eu3+ or Tb3+ ions (95SrF2·5LnF2), as well as co-doped with both the ions (95SrF2·Eu4Tb) were synthesized for comparison. Then, glass-ceramics consisting of the mono-doped or di-doped SrF2 nanocrystals incorporated into amorphous silica (90SiO2·9.5SrF2·0.5Ln or 90SiO2·9.5SrF2·0.1EuF3·0.4Tb) were prepared by sol-gel procedure. The studied materials were characterized by XRD, while the glass-ceramics additionally by SEM and BSE (backscattered electrons) techniques. Luminescence emission and excitation spectra of the doubly doped materials exhibited presence of such effects as simultaneous independent excitation and energy transfer Tb3+ → Eu3+ as well as cross-relaxation in the materials with Tb3+ dopant. Tb3+ lifetime in 90SiO2·9.5SrF2·0.5EuF3·0.4TbF3 was distinctly reduced compared to 90SiO2·9.5SrF2·0.5TbF3 probably by the Tb3+ → Eu3+ energy transfer. Owing to blue emission of SiO2, green light emitted by Tb3+ and red one by Eu3+ the 90SiO2·9.5SrF2·0.5EuF3·0.4TbF3 nano-glass-ceramics can play a white phosphor role.
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ABSORPCJA, METABOLIZM I ROLA BIOLOGICZNA KWASÓW NUKLEINOWYCH OBECNYCH W ŻYWNOŚCI
- Joanna Głazowska
- Urszula Stankiewicz
- Agnieszka Bartoszek-Pączkowska
Kwasy nukleinowe należą do niedocenianych składników żywności, szczególnie surowej lub nisko przetworzonej. W niniejszej publikacji skupiono się na omówieniu przemian, jakim podlegają kwasy nukleinowe w przewodzie pokarmowym człowieka, procesie absorpcji nukleotydów oraz nukleozydów z przewodu pokarmowego, a także przedstawiono podstawowe etapy ich metabolizmu w komórkach organizmu. Produkty trawienia kwasów nukleinowych stanowią źródło ważnych cząsteczek sygnalizacyjnych i prekursorowych, mogą być również ponownie wykorzystane do budowy nowych cząsteczek kwasów nukleinowych w organizmie człowieka. W ostatnich latach największe zainteresowanie wzbudzają niskocząsteczkowe kwasy nukleinowe, takie jak niekodujące RNA (ncRNA), także obecne w żywności. Sugeruje się możliwość oddziaływania pokarmowego ncRNA na poziomie regulacji ekspresji genów w komórkach przewodu pokarmowego, a być może i innych tkankach konsumenta. Zarówno wartość odżywcza, jak i wpływ spożywanych kwasów nukleinowych na funkcjonowanie genomu i transkryptomu wskazują na konieczność rozpatrywania żywienia człowieka w kontekście nutrigenomiki.
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Accelerated multi-objective design optimization of antennas by surrogate modeling and domain segmentation
- Sławomir Kozieł
- Adrian Bekasiewicz
- Qingsha Cheng
- Song. Li
Multi-objective optimization yields indispensable information about the best possible design trade-offs of an antenna structure, yet it is challenging if full-wave electromagnetic (EM) analysis is utilized for performance evaluation. The latter is a necessity for majority of contemporary antennas as it is the only way of achieving acceptable modeling accuracy. In this paper, a procedure for accelerated multi-objective design of antennas is proposed that exploits fast data-driven surrogates constructed at the level of coarse-discretization EM simulations, multi-objective evolutionary algorithm to yield an initial approximation of the Pareto set, and response correction methods for design refinement (i.e., elevating the selected Pareto-optimal designs to the highfidelity EM simulation model level). To reduce the computational cost of setting up the surrogate, the relevant part of the design space (i.e., the part containing the Pareto front) is firstidentified througha series of single-objective optimization runs and subsequently represented by a set of adjacent compartments with separate surrogatemodels established within them. This segmentation processdramatically reduces the number of training samples necessary to build an accurate model thus limiting the overall optimization cost.Our approach is demonstrated using a UWB monopole antenna and compared to a state-of-the-art surrogate-assistedtechnique that does not use domain segmentation.
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Accuracy of a low-cost autonomous hexacopter platforms navigation module for a photogrammetric and environmental measurements
- Paweł Burdziakowski
- Katarzyna Bobkowska
A photogrammetry and environmental measurements from an unmanned aerial vehicle (UAV) are a low-cost alternative for a traditional aerial photogrammetry. A commercial off-the-shelf products (COTS) offers a variety of cheap components that a suitable to be used on board a UAV. In this paper a low-cost navigation module based on Ublox NEO-M8N GPS and Pixhawk flight controller have been described, as a main extrinsic parameters source for aerial photogrammetry, mounted on autonomous hexacopter platform. An accuracy of navigation data logged during the drone flight by a flight controllers navigation module has been compared with geodetic reference measurement. A man controlled flight modes has been tested during a drones loitering above designated point. Due to the situation that, a variety of active electronic, radio transmitters, navigation, inertial and magnetic sensors, are to be placed on a relatively small hexarotor platform with high current power system, overall interference between electronics have to be considered. Paper presents results of navigation module fine tuning process and some technical issues solved during research, that caused an accuracy improvement and interference diminishing. Detailed results are presented and concluded as for the accuracy of navigation based on cheap GPS module is satisfied for execution an autonomous photogrammetry and environmental tasks.