2013 - Repozytorium publikacji - Politechnika Gdańska

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Biochemical characteristic of biofilm of uropathogenic Escherichia coli Dr+ strains.
- Beata Zalewska-Piątek
- Sabina Wilkanowicz
- Piotr Bruździak
- Rafał Piątek
- Józef Kur
2013 Pełny tekst MICROBIOLOGICAL RESEARCH
Urinary tract infections caused by Escherichia coli are very common health problem in the developed countries. The virulence of the uropathogenic E. coli Dr+ IH11128 is determined by Dr fimbriae, which are homopolymeric structures composed of DraE subunits with the DraD protein capping the fiber. In this study, we have analyzed the structural and biochemical properties of biofilms developed by E. coli strains expressing Dr fimbriae with or without the DraD tip subunit and the surface-exposed DraD protein. We have also demonstrated that these E. coli strains form biofilms on an abiotic surface in a nutrient-dependent fashion. We present evidence that Dr fimbriae are necessary during the first stage of bacterial interaction with the abiotic surface. In addition, we reveal that the DraD alone is also sufficient for the initial surface attachment at an even higher level than Dr fimbriae and that chloramphenicol is able to reduce the normal attachment of the analyzed E. coli. The action of chloramphenicol also shows that protein synthesis is required for the early events of biofilm formation. Additionally, we have identified reduced exopolysaccharide coverage in E. coli that express only Dr fimbrial polyadhesins at the cell surface with or without the DraD capping subunit.
BIODIVERSITY OF YEASTS ISOLATED FROM CAVES OF THE POLISH TATRA MOUNTAINS, AND ITS BIOTECHNOLOGICAL POTENTIAL
- Marta Wanarska
- Ewa Zienkiewicz
- Hubert Cieśliński
- Anna Pawlak-Szukalska
- Ewelina Krajewska
- Monika Wicka-Grochocka
- Józef Kur
2013 Postępy Mikrobiologii
Most of the Earth’s biosphere is permanently or periodically exposed to temperatures below 5°C. Cold habitats harbor a wide diversity of psychrophiles. For many years Antarctica has been the geographic area preferred for studying the diversity and properties of cold-adapted microorganisms. However, the non-Antarctic habitats like Himalayas or Alps are also extensively explored. The Tatra Mountains are Europe’s second-highest massif after the Alps. The Polish part of the Tatras has tens caves. The microclimatic conditions in the Tatra caves are very severe, with temperature ranging from below freezing to a maximum of 6°C. AIM. The aim of this study was isolation and characterization of yeasts that inhabit selected caves of the Polish Tatras. MATERIALS AND METHODS. Samples of water were collected in the Zimna Cave, Wielka Śnieżna Cave, Miętusia Cave and Kasprowa Niżnia Cave. Yeasts were isolated by growing on agar plates supplemented with ampicillin and chloramphenicol. The effect of temperature was examined at 5-37°C. Assimilation of carbon and nitrogen compounds were tested at 18°C. The proteolytic activity was examined on agar plates containing milk. Other enzymatic activities were tested using API ZYM. Isolates were identified by analysis of D1/D2 sequences of 26S rDNA. RESULTS. Nine yeast strains were isolated from water samples collected in four Tatra caves. Six of them were considered as psychrotolerants. Only one cold-adapted isolate belongs to the ascomycetous yeasts and it was classified as Candida sake. The psychrotolerant basidiomycetous yeasts were identified as Guehomyces pullulans, Rhodotorula glutinis or Cystofilobasidium capitatum. Two yeast strains had biotechnological potential. R. glutinis exhibited high proteolytic activity, whereas G. pullulans was able to produce β-galactosidase and α-amylase. CONCLUSIONS. The Tatra Mountains are the geographic region useful for studying the diversity and properties of cold-adapted microorganisms. The isolated strains can have biotechnological potential, e.g. as sources of cold-active enzymes.
Biofilm formation and its regulation in Staphylococcus aureus
- Marta Schielmann
2013
Infections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and genotypic methods. Test performed in vitro are not in agreement with the results showing the occurrence of genes involved in biofilm production. Moreover genotypic methods are focused mainly only on the amplification of two genes of the icaADBC operon, icaA and icaD genes. More efforts should be devoted to identify the regulatory proteins of biofilm production such as SarA, Agr, Bap or alternative sigma factor δB. Getting knowledge about regulatory mechanism of biofilm formation could result in finding novel molecular target for antibacterial therapy, especially infection caused by staphylococci.
Biofumigacja jako przyjazna środowisku technologia ochrony roślin
- Tadeusz Pilipczuk
- Anna Piekarska
- Barbara Kusznierewicz
- Agnieszka Bartoszek-Pączkowska
- Jacek Namieśnik
2013 Analityka: Nauka i Praktyka
Wobec rosnącej świadomości co do szkodliwości syntetycznych pestycydów, biofumigacja może stanowić alternatywną metodykę ochrony roślin. Proces ten polega na wykorzystaniu naturalnych związków antybiologicznych, zawartych m.in. w roślinach z rodziny kapustowatych, do zwalczania agrofagów. Do oceny skuteczności tej przyjaznej środowisku technologii ochrony roślin niezbędna jest znajomość metodyk oznaczania aktywności enzymatycznej mirozynazy, enzymu katalizującego hydrolizę glukozynolanów, oraz produktów ich rozpadu o potencjale biofumigacyjnym, takich jak izotiocyjaniany oraz związki indolowe.
Biokonwersja gliceryny odpadowej do wodoru przez bakterie Enterobacter aerogenes
- Paweł Lewandowski
- Iwona Hołowacz
- Donata Konopacka-Łyskawa
- Renata Tomczak-Wandzel
- Grzegorz Boczkaj
- Joanna Głazowska
- Marian Antoni Kamiński
2013
Badano możliwości produkcji wodoru w procesie ciemnej fermentacji beztlenowej z pożywki tioglikolanowej z dodatkiem gliceryny przez bakterie Enterobacter aerogenes. Przetestowano stanowisko umożliwiające prowadzenie fermentacji, odbiór i analitykę produktów lotnych i medium ciekłego. Uzyskano dobrą powtarzalność doświadczeń. Stwierdzono, że gliceryna jest trudniej przyswajalna przez bakterie niż np. glukoza, stąd ilość gazu powstającego podczas fermentacji gliceryny była około dwukrotnie niższa niż podczas „fermentacji testowej” ze standardową pożywką. Aby uzyskać wyższy stopień konwersji gliceryny do wodoru oraz zwiększenie szybkości wytwarzania wodoru potrzebne są badania nad adaptacją w/w szczepu bakterii od pożywki standardowej do bogatej w glicerynę, a także nad optymalizacją parametrów operacyjnych procesu.
Biological hazards in low noise, poroelastic road surfaces
- Beata Świeczko-Żurek
2013
Drainage pavements as well as poroelastic pavements ("PERS") are a very efficient way to decrease traffic noise by reducing tire/road noise at the source as well as noise propagation over the road surface. Many noise generating mechanisms at the tire/road interface are very much reduced by road porosity (pipe resonance, "air pumping", Helmholtz resonance and the horn effect). A well-designed porous or poroelastic road surface may decrease tire/road noise by as much as 10dB in comparison to a "typical" road surface Stone Mastics Asphalt 12 mm. There are also problems and potential problems associated with porous surfaces. Probably the most important one is clogging. Most research on clogging concentrates only on its effect on noise reduction potential. However, there are some concerns related to biohazards caused by formation of biofilm resulting from adhesis and the growth of microorganisms on the surface and in the porous structure. In practice, all surfaces may be subjected to colonizing processes performed by live microorganisms. The presence of humidity in voids is particularly favora-ble for the development of microorganisms. Owing to that, they can easily adjust to different extreme surrounding conditions. The paper describes certain tests of microorganism for-mation on poroelastic noise reducing road surfaces in Europe.
Biosurfactants - Biodegradability, toxicity, efficiency in comparison with synthetic surfactants
- Ilona Edyta Kłosowska-Chomiczewska
- Krystyna Mędrzycka
- Elena Karpenko
2013
Biosurfactants are natural surface active agents produced by variety of bacteria yeast and fungi. A review of their biodegradability, toxicity and efficiency is presented in order to compare their properties with synthetic surfactants. Due to their high efficiency in many applications and environmentally friendly character we tried to apply them in oil refining process. The efficiency of biosurfactants in this application was very high and reached up to 99%.
Bis(ammonium) Zoledronate Dihydrate
- Małgorzata Sikorska
- Jarosław Chojnacki
2013 Pełny tekst Journal of Applied Crystallography
Neutralization of 2-(1-imidazole)-1-hydroxyl-1,1`-ethylidenediphosphonic acid (zoledronic acid) by an excess of ammonia yielded bis(ammonium) zoledronate dihydrate, {C5H8N2O7P2 2−, 2(H4N+), 2(H2O)}. The product is readily soluble in water and forms monocrystals for which the X-ray structural analysis was carried out. The zoledronic anion is of double negative charge due to deprotonation of three P–OH groups and protonation of the nitrogen in the imidazole ring.The structure is stabilized by extensive network of N–H⋅ ⋅ ⋅O andO–H⋅ ⋅ ⋅O hydrogen bonds expanding through the crystal in plane (002).The imidazole ring is involved in pi-pi stacking interactions with its symmetry equivalents related by inversion centers at (1 0 0) and (1 1/2 0), with distances between centroids (Cg–Cg) of 3.819 (2) and 3.881 (2) A, respectively
Bis(diethylamido-[kappa]N)(diethylamine-[kappa]N)bis(2,6-diisopropylphenylamido-[kappa]N)zirconium(IV)
- Mateusz Zauliczny
- Rafał Grubba
- Łukasz Ponikiewski
- Jerzy Pikies
2013 Pełny tekst ACTA CRYSTALLOGRAPHICA SECTION E-STRUCTURE REPORTS ONLINE
In the title compound, [Zr(C12H18N)2(C4H10N)2(C4H11N)] or [Zr(HNC6H3iPr2)2(NEt2)2(HNEt2)], which was obtained by the reaction of Zr(NEt)4 with iPr2C6H3NH2, the Zr IV atom is in a trigonal–bipiramidal geometry in which the N atoms from two iPr2C6H3NH and one NEt2 ligand occupy the equatorial positions, and the N atoms of an NEt2 and an Et2NH ligand occupy the apical positions. An intramolecular N—HN contact occurs. There are two independent molecules in the asymmetric unit.
Bis(diisopropylammonium) thiosulfate and bis(tert-butylammonium) thiosulfate
- Andrzej Okuniewski
- Jarosław Chojnacki
- Katarzyna Kazimierczuk
- Barbara Becker
2013 Pełny tekst ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS
Two new dialkylammonium thiosulfates, namely bis(diisopropylammonium) thiosulfate, 2C6H16N+S2O3 2-, (I), and bis(tert-butylammonium) thiosulfate, 2C4H12N+S2O3 2-, (II), have been characterized. The secondary ammonium salt (I) crystallizes with Z = 4, while the primary ammonium salt (II), with more hydrogen-bond donors, crystallizes with Z = 8 and a noncrystallographic centre of inversion. In both salts, the organic cations and thiosulfate anions are linked within extensive N—H...O and N—H...S hydrogen-bond networks, forming extended two-dimensional layers. Layers are parallel to (101) in (I) and to (002) in (II), and have a polar interior and a nonpolar hydrocarbon exterior. The layered structure and hydrogen-bond motifs observed in (I) and (II) are similar to those in related ammonium sulfates.
Block-based Representation of Application Execution on Modern Parallel Systems
- Paweł Czarnul
2013
The chapter presents how to model execution of a parallel computational application that is to be executed in a large-scale parallel or distributed environment with potentially thousands to millions of execution units. The representation uses pre- viously attributes and factors representative of modern high performance systems including multicore CPUs, GPUs, dedicated accelerators such as Intel Phi.
Blogi jako platforma komunikowania na przykładzie blogów uczelnianych
- Ewa Hope
2013 Pełny tekst Studia Ekonomiczne. Zeszyty Naukowe Uniwersytetu Ekonomicznego w Katowicach
W artykule stawiam tezę o istotnej roli blogów jako niezwykle skutecznej platformie komunikacyjnej w dobie konwergencji mediów. Szczególnie ważną rolę to specyficzne narzędzie komunikacyjne może odegrać w działaniach public relations uczelni: jako narzędzie za pomocą którego można najpełniej realizować funkcje uczelni i te, które realizuje najwyższy poziom rozwoju PR - w komunikacji dwukierunkowej symetrycznej zgodnie z modelem Hunta/Gruniga. Tezę potwierdzam analizą literatury przedmiotu ostatnich lat dotyczącej nowych mediów, stron internetowych uczelni publicznych w Polsce, analizą wdrożenia/funkcjonowania blogów na Wydziale Zarządzania i Ekonomii Politechniki Gdańskiej. W artykule proponuję też - jako wnioski - zasady budowania strategii poprawy komunikacji instytucji edukacyjnych poprzez wdrożenie takiego narzędzia jak blogi.
Bloki oporowe przy przewodach ciśnieniowych
- Ziemowit Suligowski
2013
Bloki oporowe na przewodach. Stabilizacja położenia. Zabezpieczenie przed przesuwaniem. Podstawowe konstrukcje. Zasady stosowania.
BLOOD PRESSURE ESTIMATION BY MEANS OF A JOINT IMPEDANCE– PHOTOPLETYSMOGRAPHIC METHOD
- Krzysztof Czuszyński
- Jerzy Wtorek
2013
The knowledge of patient’s day to day blood pressure changes is invaluable to physicians for both diagnostics and health monitoring reasons. Constant observation of the pressure throughout a day would provide even more valuable clinical information. A convenient non-invasive methods of blood pressure estimation for monitoring purposes are widely proposed. This work shows a statistical approach to the problem and considers estimation of BP using two different parameters related to arterial elasticity. Multiple linear regression analysis with different sets of explanatory variables, separate for systolic and diastolic phase, has been performed. A medical device CNAP 500 Monitor was utilized as a reference measurement system. Two statistical models based on different sets of explanatory variables were examined. A comparison of the results received from these models are discussed in the paper.
Błedy projektowe i wykonawcze przyczyną usterek tarasu
- Maciej Niedostatkiewicz
2013 Dachy
W artykule przedstawiono opis usterek stropu nad ostatnią kondtygnacją wielorodzinnego budynku mieszkalnego, w pozimi którego zaprojektowany i wykonany został taras. Przedstawiono analizę wpływu przyjetych rozwiąan projektowych i wykonawczych na stan techniczny budynku oraz zaproponowano koncepcje rozwiązania projektowego przebudowy tarasu
Błędy cyfrowej rejestracji prędkości obrotowej z wykorzystaniem przetwornika obrotowo-impulsowego
- Dariusz Świsulski
2013 Pełny tekst Zeszyty Naukowe Wydziału Elektrotechniki i Automatyki Politechniki Gdańskiej
Przedstawiono zasadę działania przetwornika obrotowo-impulsowego w zastosowaniu do pomiaru prędkości obrotowej. Omówiono metody rejestracji on-line sygnału impulsowego z przetwornika obrotowo-impulsowego. W metodach tych prędkość obrotowa wyznaczana jest na podstawie ostatniego przedziału miedzyimpulsowego lub na podstawie dwóch ostatnich przedziałów miedzyimpulsowych z ekstrapolacji. Dla sinusoidalnej zmiany prędkości udowodniono, że metoda, przy której uzyskujemy mniejszą wartość błędu pomiaru, zależy od zmian prędkości oraz parametrów pomiaru.
Book review: Simulation-Driven Design Optimisation and Modelling for Microwave Engineering
- Stanisław Szczepański
2013 International Journal of Mathematical Modelling and Numerical Optimisation
Celem książki jest przedstawienie aktualnego stanu badań dotyczących projektowania układów mikrofalowych poprzez modelowanie i optymalizacje wspomagane symulacjami elektromagnetycznymi. Grupa międzynarodowych ekspertów zajmujących się rożnymi aspektami komputerowo wspomaganego projektowania układów mikrofalowych, podsumowuje i dokonuje przeglądu ostatnich osiągnięć w tej dziedzinie oraz przedstawia szereg praktycznych zastosowań. Tematyka zawarta w niniejszej pracy obejmuje techniki optymalizacji (klasyczne oraz wykorzystujące modele zastępcze, ang. surrogate models), metody szybkiego strojenia układów oparte o symulacje elektromagnetyczne, metody odwzorowania przestrzeni (ang. space mapping), a także wybrane techniki modelowania, w tym sztuczne sieci neuronowe oraz kriging. Przedstawiono także zastosowania wybranych technik do rozwiazywania problemów projektowania filtrów, anten, oraz układów aktywnych. Książka zawiera również podstawy w zakresie technik numerycznego modelowania i optymalizacji.
Botanical and geographical origin characterization of polish honeys by headspace SPME-GCxGC-TOFMS
- Tomasz Dymerski
- Tomasz Chmiel
- Ahmed Mostafa
- Magdalena Śliwińska
- Paulina Wiśniewska
- Waldemar Wardencki
- Jacek Namieśnik
- Tadeusz Gorecki
2013 CURRENT ORGANIC CHEMISTRY
Volatile organic compounds (VOCs) composition of Polish honeys obtained from various geographical regions was studied. Headspace solid phase microextraction (HS-SPME) using Divinylbenzene/Carboxen/Polydimethylsiloxane-coated fibers was used in combination with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC× GC-TOFMS) because of the complexity of the samples. Acacia, linden, rapeseed, buckwheat and honeydew honeys were studied in this project.A total of 329 compounds were identified during the investigations. Positive identification of 82 compounds was achieved using analytical standards, while tentative identification of 247 compounds was based on comparison of deconvoluted mass spectra and linear temperature programmed retention indices (LTPRI) with NIST 2005 library and literature values, respectively. Many VOCs identified in Polish honey samples were commonly present in honeys from other geographical regions of Europe (e.g. Italy, Corsica). They included certain acyclic and cyclic alkanes, acyclic and cyclic alkenes, aromatic hydrocarbons, oxygenated aromatic compounds, alcohols, aldehydes, ketones, esters, ethers, nitriles, organic sulphides, phenolic compounds and terpenes. GC× GC-TOFMS profiles for the same types of honey samples were remarkably similar. Eight botanic discriminants were selected among all compounds. Their identity was confirmed by comparison with authentic standards. Furthermore, possible geographical discriminants for Polish honeys were identified, including 19 for acacia, 3 for linden, 3 for rapeseed and buckwheat and 6 for honeydew honeys.
Bridging challenges of clinical decision support systems with a semantic approach. A case study on breast cancer
- Edward Szczerbicki
- Cesar Sanin
- Carlos Toro
2013 PATTERN RECOGNITION LETTERS
The integration of Clinical Decision Support Systems (CDSS) in nowadays clinical environments has not been fully achieved yet. Although numerous approaches and technologies have been proposed since 1960, there are still open gaps that need to be bridged. In this work we present advances from the established state of the art, overcoming some of the most notorious reported difficulties in: (i) automating CDSS, (ii) clinical workflow integration, (iii) maintainability and extensibility of the system, (iv) timely advice, (v) evaluation of the costs and effects of clinical decision support, and (vi) the need of architectures that allow the sharing and reusing of CDSS modules and services. In order to do so, we introduce a new clinical task model oriented to clinical workflow integration, which follows a federated approach. Our work makes use of the reported benefits of semantics in order to fully take advantage of the knowledge present in every stage of clinical tasks and the experience acquired by physicians. In order to introduce a feasible extension of classical CDSS, we present a generic architecture that permits a semantic enhancement, namely Semantic CDSS (S-CDSS). A case study of the proposed architecture in the domain of breast cancer is also presented, pointing some highlights of our methodology.
Bringing Common Sense to WordNet with a Word Game
- Jacek Rzeniewicz
- Julian Szymański
2013
We present a tool for common sense knowledge acquisition in form of a twenty questions game. The described approach uses WordNet dictionary, which rich taxonomy allows to keep cognitive economy and accelerate knowledge propagation, although sometimes inferences made on hierarchical relations result in noise. We extend the dictionary with common sense assertions acquired during the games played with humans. The facts added to the knowledge base use eight new relation types. After 700 games played in the animals domain the average number of assertions per concept raised over 891%. Evaluation of the newly acquired facts indicates high quality of knowledge captured using proposed approach.